Optomechanical measurement of the role of lamins in whole cell deformability
Thorsten Kolb
Department of Physics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestrasse 91, 91052 Erlangen, Germany
Division of Molecular Genetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
Search for more papers by this authorJulia Kraxner
Department of Physics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestrasse 91, 91052 Erlangen, Germany
Search for more papers by this authorKai Skodzek
Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh, EH14 4AS UK
Search for more papers by this authorMichael Haug
Department of Physics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestrasse 91, 91052 Erlangen, Germany
Search for more papers by this authorDean Crawford
Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh, EH14 4AS UK
Search for more papers by this authorKendra K. Maaß
Division of Molecular Genetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
Search for more papers by this authorKaterina E. Aifantis
Lab of Mechanics and Materials, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Department of Civil Engineering-Engineering Mechanics, University of Arizona, Tuscon, Arizona, 85721
Search for more papers by this authorCorresponding Author
Graeme Whyte
Department of Physics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestrasse 91, 91052 Erlangen, Germany
Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh, EH14 4AS UK
Search for more papers by this authorThorsten Kolb
Department of Physics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestrasse 91, 91052 Erlangen, Germany
Division of Molecular Genetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
Search for more papers by this authorJulia Kraxner
Department of Physics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestrasse 91, 91052 Erlangen, Germany
Search for more papers by this authorKai Skodzek
Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh, EH14 4AS UK
Search for more papers by this authorMichael Haug
Department of Physics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestrasse 91, 91052 Erlangen, Germany
Search for more papers by this authorDean Crawford
Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh, EH14 4AS UK
Search for more papers by this authorKendra K. Maaß
Division of Molecular Genetics, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany
Search for more papers by this authorKaterina E. Aifantis
Lab of Mechanics and Materials, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Department of Civil Engineering-Engineering Mechanics, University of Arizona, Tuscon, Arizona, 85721
Search for more papers by this authorCorresponding Author
Graeme Whyte
Department of Physics, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestrasse 91, 91052 Erlangen, Germany
Institute of Biological Chemistry, Biophysics and Bioengineering, Heriot-Watt University, Edinburgh, EH14 4AS UK
Search for more papers by this authorAbstract
There is mounting evidence that the nuclear envelope, and particularly the lamina, plays a critical role in the mechanical and regulation properties of the cell and changes to the lamina can have implications for the physical properties of the whole cell. In this study we demonstrate that the optical stretcher can measure changes in the time-dependent mechanical properties of living cells with different levels of A-type lamin expression. Results from the optical stretcher shows a decrease in the deformability of cells as the levels of lamin A increases, for cells which grow both adherently and in suspension. Further detail can be probed by combining the optical stretcher with fluorescence microscopy to investigate the nuclear mechanical properties which show a larger decrease in deformability than for the whole cell.
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