Concise Report
Characterization of HpnG as a Purine Nucleoside Phosphorylase in Bacteriohopanepolyol Biosynthesis†
Xinhui Li,
Xiaoyu Zhu,
Yuting Zhong,
Wei Zhang,
Fener Chen,
Wenning Wang,
Wei Ding,
Qi Zhang,
Xinhui Li
Department of Chemistry, Fudan University, Shanghai, 200433 China
These authors contributed equally to this work.
Search for more papers by this authorXiaoyu Zhu
Department of Chemistry, Fudan University, Shanghai, 200433 China
These authors contributed equally to this work.
Search for more papers by this authorYuting Zhong
Department of Chemistry, Fudan University, Shanghai, 200433 China
State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China
Search for more papers by this authorWei Zhang
Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, Gansu, 73000 China
Search for more papers by this authorFener Chen
Department of Chemistry, Fudan University, Shanghai, 200433 China
Search for more papers by this authorWenning Wang
Department of Chemistry, Fudan University, Shanghai, 200433 China
Search for more papers by this authorWei Ding
State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China
Search for more papers by this authorCorresponding Author
Qi Zhang
Department of Chemistry, Fudan University, Shanghai, 200433 China
E-mail: [email protected]Search for more papers by this authorXinhui Li,
Xiaoyu Zhu,
Yuting Zhong,
Wei Zhang,
Fener Chen,
Wenning Wang,
Wei Ding,
Qi Zhang,
Xinhui Li
Department of Chemistry, Fudan University, Shanghai, 200433 China
These authors contributed equally to this work.
Search for more papers by this authorXiaoyu Zhu
Department of Chemistry, Fudan University, Shanghai, 200433 China
These authors contributed equally to this work.
Search for more papers by this authorYuting Zhong
Department of Chemistry, Fudan University, Shanghai, 200433 China
State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China
Search for more papers by this authorWei Zhang
Key Laboratory of Extreme Environmental Microbial Resources and Engineering of Gansu Province, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou, Gansu, 73000 China
Search for more papers by this authorFener Chen
Department of Chemistry, Fudan University, Shanghai, 200433 China
Search for more papers by this authorWenning Wang
Department of Chemistry, Fudan University, Shanghai, 200433 China
Search for more papers by this authorWei Ding
State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240 China
Search for more papers by this authorCorresponding Author
Qi Zhang
Department of Chemistry, Fudan University, Shanghai, 200433 China
E-mail: [email protected]Search for more papers by this author†
Dedicated to the Special Issue of Emerging Investigators in 2024.
Comprehensive Summary
HpnG plays a crucial role in the production of ribosylhopane, a key intermediate in the biosynthesis of bacteriohopanepolyol. Despite early extensive studies, the precise function of HpnG has remained elusive. Here, we report functional characterization of HpnG as a purine nucleoside phosphorylase, which converts adenosylhopane to phosphoribosylhopane in the presence of phosphate. HpnG demonstrates broad substrate specificity and impressive stability, making it a valuable enzymatic tool for applications in nucleoside processing and related biotechnology.
Supporting Information
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Appendix S1: Supporting Information |
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