DNA-Based Architectures for in situ Target Biomolecule Analysis in Confined Nano-space†
Xiaoxue Hu
Shenzhen Research Institute, Nanjing University, Shenzhen, Guangdong, 518000 China
College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorYide Huang
College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorHao Yin
College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorLizhi Dai
College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorCorresponding Author
Ye Tian
Shenzhen Research Institute, Nanjing University, Shenzhen, Guangdong, 518000 China
College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing, Jiangsu, 210093 China
E-mail: [email protected]Search for more papers by this authorXiaoxue Hu
Shenzhen Research Institute, Nanjing University, Shenzhen, Guangdong, 518000 China
College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorYide Huang
College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorHao Yin
College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorLizhi Dai
College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorCorresponding Author
Ye Tian
Shenzhen Research Institute, Nanjing University, Shenzhen, Guangdong, 518000 China
College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science, Chemistry and Biomedicine Innovation Center, Nanjing University, Nanjing, Jiangsu, 210093 China
E-mail: [email protected]Search for more papers by this author#These authors contributed equally to this work
†Dedicate to the Special Issue of In Situ Target Biomolecule Analysis in Confined Nanospace.
Abstract
In situ target biomolecule analysis is of great significance for real-time monitoring and regulation of endogenous biomarkers and elementary biomolecules in vivo. Gratifyingly, the rapid evolution of structural DNA nanotechnology during past decades has established an appealing toolbox for biological analysis and medical detection. The modulated self-assembly and underlying canonical Watson-Crick base-pairing rules provide possibilities for accurate controlling of the topologies and functions of obtained nanomaterials. The probes composed of diverse DNA nanostructures and DNA-nanoparticle complexes can create a confined space, which increases target accessibility and improves probe stability, sensitivity and specificity. In this minireview, we retrospect the research progress of in-situ biomolecular analysis based on DNA nanostructures for intracellular and in vivo biosensors in confined space. The characteristics of distinct DNA nanomaterials are first introduced, and then the fundamentals of biosensing process of designed DNA nanostructures are emphasized. Moreover, we elucidate our perspective over the challenges of this field and discuss the potential directions of this kind of application-oriented fabrication technique.
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