Intracellular Wireless Analysis of Single Cells by Bipolar Electrochemiluminescence Confined in a Nanopipette
Yuling Wang
State Key Laboratory of Analytical Chemistry for Life and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093 China
These authors contributed equally to this work.
Search for more papers by this authorRong Jin
State Key Laboratory of Analytical Chemistry for Life and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093 China
These authors contributed equally to this work.
Search for more papers by this authorCorresponding Author
Prof. Neso Sojic
Bordeaux INP, Institute of Molecular Science (ISM), and CNRS UMR 5255, University of Bordeaux, 33607 Pessac, France
Search for more papers by this authorCorresponding Author
Prof. Dechen Jiang
State Key Laboratory of Analytical Chemistry for Life and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorProf. Hong-Yuan Chen
State Key Laboratory of Analytical Chemistry for Life and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorYuling Wang
State Key Laboratory of Analytical Chemistry for Life and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093 China
These authors contributed equally to this work.
Search for more papers by this authorRong Jin
State Key Laboratory of Analytical Chemistry for Life and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093 China
These authors contributed equally to this work.
Search for more papers by this authorCorresponding Author
Prof. Neso Sojic
Bordeaux INP, Institute of Molecular Science (ISM), and CNRS UMR 5255, University of Bordeaux, 33607 Pessac, France
Search for more papers by this authorCorresponding Author
Prof. Dechen Jiang
State Key Laboratory of Analytical Chemistry for Life and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorProf. Hong-Yuan Chen
State Key Laboratory of Analytical Chemistry for Life and School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu, 210093 China
Search for more papers by this authorDedicated to the 100th anniversary of the School of Chemistry and Chemical Engineering, Nanjing University
Abstract
The inside walls of a nanopipette tip are decorated by a Pt deposit that is used as an open bipolar electrochemiluminescence (ECL) device to achieve intracellular wireless electroanalysis. The synergetic actions of nanopipette and of bipolar ECL lead to the spatial confinement of the voltage drop at the level of the Pt deposit, which generates ECL emission from luminol. The porous structure of Pt deposit permits the electrochemical transport of intracellular molecules into the nanopipette that is coupled with enzymatic reactions. Thus, the intracellular concentrations of hydrogen peroxide or glucose are measured in vivo as well as the intracellular sphingomyelinase activity. In comparison with the classic bipolar ECL, the remarkably low potential applied in our approach is restricted inside the nanopipette and it minimizes the potential bias of the voltage on the cellular activity. Accordingly, this wireless ECL approach provides a new direction for analysis of single living cells.
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