Chemical Synthesis of Diubiquitin-Based Photoaffinity Probes for Selectively Profiling Ubiquitin-Binding Proteins
Jun Liang
Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and University of Science and Technology of China, Hefei, 230026 China
Search for more papers by this authorLin Zhang
MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorXiang-Long Tan
Department of Chemistry, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorYun-Kun Qi
Department of Chemistry, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorShan Feng
MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorProf. Haiteng Deng
MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorProf. Yijing Yan
Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and University of Science and Technology of China, Hefei, 230026 China
Search for more papers by this authorProf. Ji-Shen Zheng
Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and University of Science and Technology of China, Hefei, 230026 China
Search for more papers by this authorProf. Lei Liu
Department of Chemistry, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorCorresponding Author
Prof. Chang-Lin Tian
Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and University of Science and Technology of China, Hefei, 230026 China
Search for more papers by this authorJun Liang
Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and University of Science and Technology of China, Hefei, 230026 China
Search for more papers by this authorLin Zhang
MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorXiang-Long Tan
Department of Chemistry, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorYun-Kun Qi
Department of Chemistry, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorShan Feng
MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorProf. Haiteng Deng
MOE Key Laboratory of Bioinformatics, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorProf. Yijing Yan
Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and University of Science and Technology of China, Hefei, 230026 China
Search for more papers by this authorProf. Ji-Shen Zheng
Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and University of Science and Technology of China, Hefei, 230026 China
Search for more papers by this authorProf. Lei Liu
Department of Chemistry, Tsinghua University, Beijing, 100084 China
Search for more papers by this authorCorresponding Author
Prof. Chang-Lin Tian
Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and University of Science and Technology of China, Hefei, 230026 China
Search for more papers by this authorAbstract
Biochemical studies of cellular processes involving polyubiquitin have gained increasing attention. More tools are needed to identify ubiquitin (Ub)-binding proteins. We report diazirine-based photoaffinity probes that can capture Ub-binding proteins in cell lysates, and show that diazirines are preferable to aryl azides as the photo-crosslinking group, since they decrease non-selective capture. Photoaffinity probes containing at least two Ub units were required to effectively capture Ub-binding proteins. Different capture selectivity was observed for probes containing diubiquitin moieties with different types of linkages, thus indicating the potential to develop linkage-dependent probes for selectively profiling Ub-binding proteins under various cellular conditions.
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