Dipolar Relaxation Dynamics at the Active Site of an ATPase Regulated by Membrane Lateral Pressure
Elisabeth Fischermeier
Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstrasse 400, 01328 Dresden, Germany
Technische Universität Dresden, Biotechnology Center, Tatzberg 47-49, 01307, Dresden, Germany
Nationales Zentrum für Tumorerkrankungen Heidelberg, Im Neuenheimer Feld 460, 69120 Heidelberg, Germany
Search for more papers by this authorPetr Pospíšil
J. Heyrovský Inst. Physical Chemistry of the A.S.C.R. v.v.i., Prague, Czech Republic
Search for more papers by this authorAhmed Sayed
Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstrasse 400, 01328 Dresden, Germany
Technische Universität Dresden, Biotechnology Center, Tatzberg 47-49, 01307, Dresden, Germany
Institute for Experimental Physics I, Universität Leipzig, Linnéstrasse 5, 04103 Leipzig, Germany
Search for more papers by this authorMartin Hof
J. Heyrovský Inst. Physical Chemistry of the A.S.C.R. v.v.i., Prague, Czech Republic
Search for more papers by this authorMarc Solioz
University of Bern, Dept. of Clinical Pharmacology, Murtenstrasse 35, 3008 Bern, Switzerland
Search for more papers by this authorCorresponding Author
Karim Fahmy
Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstrasse 400, 01328 Dresden, Germany
Technische Universität Dresden, Biotechnology Center, Tatzberg 47-49, 01307, Dresden, Germany
Search for more papers by this authorElisabeth Fischermeier
Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstrasse 400, 01328 Dresden, Germany
Technische Universität Dresden, Biotechnology Center, Tatzberg 47-49, 01307, Dresden, Germany
Nationales Zentrum für Tumorerkrankungen Heidelberg, Im Neuenheimer Feld 460, 69120 Heidelberg, Germany
Search for more papers by this authorPetr Pospíšil
J. Heyrovský Inst. Physical Chemistry of the A.S.C.R. v.v.i., Prague, Czech Republic
Search for more papers by this authorAhmed Sayed
Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstrasse 400, 01328 Dresden, Germany
Technische Universität Dresden, Biotechnology Center, Tatzberg 47-49, 01307, Dresden, Germany
Institute for Experimental Physics I, Universität Leipzig, Linnéstrasse 5, 04103 Leipzig, Germany
Search for more papers by this authorMartin Hof
J. Heyrovský Inst. Physical Chemistry of the A.S.C.R. v.v.i., Prague, Czech Republic
Search for more papers by this authorMarc Solioz
University of Bern, Dept. of Clinical Pharmacology, Murtenstrasse 35, 3008 Bern, Switzerland
Search for more papers by this authorCorresponding Author
Karim Fahmy
Helmholtz-Zentrum Dresden-Rossendorf, Institute of Resource Ecology, Bautzner Landstrasse 400, 01328 Dresden, Germany
Technische Universität Dresden, Biotechnology Center, Tatzberg 47-49, 01307, Dresden, Germany
Search for more papers by this authorAbstract
The active transport of ions across biological membranes requires their hydration shell to interact with the interior of membrane proteins. However, the influence of the external lipid phase on internal dielectric dynamics is hard to access by experiment. Using the octahelical transmembrane architecture of the copper-transporting P1B-type ATPase from Legionella pneumophila as a model structure, we have established the site-specific labeling of internal cysteines with a polarity-sensitive fluorophore. This enabled dipolar relaxation studies in a solubilized form of the protein and in its lipid-embedded state in nanodiscs. Time-dependent fluorescence shifts revealed the site-specific hydration and dipole mobility around the conserved ion-binding motif. The spatial distribution of both features is shaped significantly and independently of each other by membrane lateral pressure.
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