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An Effective Minor Groove Binder as a Red Fluorescent Marker for Live-Cell DNA Imaging and Quantification^†

Corresponding Author

Prof. Xiaojun Peng

[email protected]

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)Search for more papers by this author

Tong Wu,

Tong Wu

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

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Jiangli Fan,

Jiangli Fan

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

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Prof. Jingyun Wang,

Prof. Jingyun Wang

School of Life Science & Biotechnology, Dalian University of Technology (China)

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Si Zhang,

Si Zhang

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

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Fengling Song,

Fengling Song

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

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Shiguo Sun,

Shiguo Sun

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

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Prof. Xiaojun Peng,

Corresponding Author

Prof. Xiaojun Peng

[email protected]

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)Search for more papers by this author

Tong Wu,

Tong Wu

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

Search for more papers by this author

Jiangli Fan,

Jiangli Fan

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

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Prof. Jingyun Wang,

Prof. Jingyun Wang

School of Life Science & Biotechnology, Dalian University of Technology (China)

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Si Zhang,

Si Zhang

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

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Fengling Song,

Fengling Song

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

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Shiguo Sun,

Shiguo Sun

State Key Laboratory of Fine Chemicals, Dalian University of Technology, 2 Linggong Road, 116024 Dalian (China)

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First published: 06 April 2011

https://doi.org/10.1002/ange.201007386

Citations: 18

^†

We thank the NSF of China (Nos. 20725621, 20876024, 21076032, 21072024, and 21006009), the National Basic Research Program of China (2009CB724706), and the Cultivation Fund of the Key Scientific and Technical Innovation Project (707016) for support of this work. We also thank Dr. Richard W. Horobin for advice on the estimation of logP values.

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Graphical Abstract

Ich seh' rot! Das zellgängige DEAB-TO-3 bindet in die kleine Furche von DNA und zeigt auch in Gegenwart von RNA eine Selektivität für Doppelstrang-DNA. Außerdem wird die rote Fluoreszenz der Verbindung durch das Bindeereignis deutlich verstärkt. Daher ist DEAB-TO-3 ein aussichtsreicher Kandidat für den hoch empfindlichen DNA-Nachweis in vitro sowie für die Bildgebung von Zellkernen und die DNA-Quantifizierung in vivo.

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Volume123, Issue18

April 26, 2011

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An Effective Minor Groove Binder as a Red Fluorescent Marker for Live-Cell DNA Imaging and Quantification^†

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An Effective Minor Groove Binder as a Red Fluorescent Marker for Live-Cell DNA Imaging and Quantification^†