Journal of Biomedical Materials Research

Osteoblast cell death on methacrylate polymers involves apoptosis

Corresponding Author

Julie E. Gough

[email protected]

School of Biomedical Sciences, E Floor, University of Nottingham, Medical School, Queen's Medical Centre, Nottingham, NG7 2UH, United Kingdom

School of Biomedical Sciences, E Floor, University of Nottingham, Medical School, Queen's Medical Centre, Nottingham, NG7 2UH, United KingdomSearch for more papers by this author

Sandra Downes,

Sandra Downes

Smith and Nephew Group Research Centre, York Science Park, Heslington, York, YO10 5DF, United Kingdom

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Julie E. Gough,

Corresponding Author

Julie E. Gough

[email protected]

School of Biomedical Sciences, E Floor, University of Nottingham, Medical School, Queen's Medical Centre, Nottingham, NG7 2UH, United Kingdom

School of Biomedical Sciences, E Floor, University of Nottingham, Medical School, Queen's Medical Centre, Nottingham, NG7 2UH, United KingdomSearch for more papers by this author

Sandra Downes,

Sandra Downes

Smith and Nephew Group Research Centre, York Science Park, Heslington, York, YO10 5DF, United Kingdom

Search for more papers by this author

First published: 28 August 2001

https://doi.org/10.1002/1097-4636(20011215)57:4<497::AID-JBM1195>3.0.CO;2-U

Citations: 48

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Abstract

The success of an implant depends on the implant–tissue interface. There are many causes of implant failure, one of which is tissue necrosis. The aim of this in vitro study was to determine whether cell death of primary human osteoblasts (implant site specific cells) occurred by apoptosis (a form of programmed cell death) on two methacrylate polymers. Cells were cultured on poly(ethyl methacrylate)/tetrahydrofurfuryl methacrylate and poly(methyl methacrylate in the form of 13-mm discs, in conditioned medium containing leachable monomer and in the presence of various concentrations of monomer itself in the culture medium. It was found that monomer and leached monomer caused apoptosis of human osteoblast cells in this system. Tetrahydrofurfuryl methacrylate monomer was found to be more toxic than currently used monomer methylmethacrylate. Preincubation of polymers in serum containing medium was found to increase the biocompatibility of the polymers. High levels of apoptosis occurred on polymer used directly after polymerization. Apoptosis levels were decreased after polymer was incubated at 60°C overnight or for 3 days. Apoptosis therefore may occur in cells at the implant site in vivo. © 2001 John Wiley & Sons, Inc. J Biomed Mater Res 57: 497–505, 2001

References

1Downes S, Dabestani M, editors. Failure of joint replacement: A biological, surgical or mechanical problem? Middlesex, UK: The Institute of Orthopaedics; 1994.
Google Scholar
2Willert HG, Ludwig J, Semlitsch M. Reaction of bone to methacrylate after hip arthroplasty. J Bone Joint Surg 1974; 56A: 1369–1382.
Google Scholar
3Kerr JFR, Wyllie AH, Currie AR. Apoptosis: A basic biological phenomenon with wide ranging implications in tissue kinetics. Br J Cancer 1972; 26: 239–257.
10.1038/bjc.1972.33
CAS PubMed Web of Science® Google Scholar
4Milligan CE, Schwartz LM. Programmed cell death during animal development. Br Med Bull 1997; 52(3): 570–590.
10.1093/oxfordjournals.bmb.a011631
Google Scholar
5Carson DA, Ribeiro JM. Apoptosis and disease. Lancet 1993; 341: 1251–1254.
10.1016/0140-6736(93)91154-E
CAS PubMed Web of Science® Google Scholar
6Falcieri E, Gobbi P, Zamai L, Vitale M. Ultrastructural features of apoptosis. Scanning Microsc 1994; 8(3): 653–666.
CAS PubMed Web of Science® Google Scholar
7Enari M, Skahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase activated DNase that degrades DNA during apoptosis and its inhibitor ICAD. Nature 1998; 391: 43–50.
10.1038/34112
CAS PubMed Web of Science® Google Scholar
8Fraser A, Evan G. A license to kill. Cell 1996; 85: 781–784.
10.1016/S0092-8674(00)81005-3
CAS PubMed Web of Science® Google Scholar
9Schulze-Osthoff K, Ferrari D, Los M, Wesselberg S, Peter ME. Apoptosis signalling by death receptors. Eur J Biochem 1998; 254(3): 439–459.
10.1046/j.1432-1327.1998.2540439.x
CAS PubMed Web of Science® Google Scholar
10Sun X-M, Snowden RT, Skilleter DN, Dinsdale D, Ormerod MG, Cohen GM. A flow cytometric method for the separation and quantitation of normal and apoptotic thymocytes. Anal Biochem 1992; 204: 351–356.
10.1016/0003-2697(92)90251-2
CAS PubMed Web of Science® Google Scholar
11McGahon AJ, Martin SJ, Bissonnette RP, Mhaboubi A, Shi Y, Mogil RJ, Nishioka WK, Green DR. The end of the (cell) line: Methods for the study of apoptosis in vitro. In: LM Schwartz, BA Osborne, editors. Methods in cell biology, Volume 46. New York: Academic Press; 1995. p 153–185.
10.1016/S0091-679X(08)61929-9
Google Scholar
12Belloc F, Dumain P, Boisseau MR, Jalloustre C, Reiffers J, Bernard P, Lacombe F. A flow cytometric method using Hoechst 33342 and propidium iodide for simultaneous cell cycle analysis and apoptosis determination in unfixed cells. Cytometry 1994; 17: 59–65.
10.1002/cyto.990170108
CAS PubMed Web of Science® Google Scholar
13Trump BF, Berezesky IK. The reactions of cells to lethal injury: Oncosis and necrosis—the role of calcium. In: RA Lockshin, Z Zakeri, JL Tilly, editors. When cells die. New York: Wiley-Liss; 1998. p. 57–96.
Google Scholar
14Poss R, Walker P, Spector M, Reilly DT, Robertson DD, Sledge CB. Strategies for improving fixation of femoral components in total hip arthroplasty. Clin Orthop Rel Res 1988; 235: 181–194.
PubMed Web of Science® Google Scholar
15Sawtell RM, Kayser MV, Downes S. A morphological assessment of bovine chondrocytes cultured on poly (ethyl methacrylate)/tetrahydrofurfuryl methacrylate. Cells Mater 1995; 5(1): 63–71.
CAS Web of Science® Google Scholar
16Reissis N, Downes S, Kayser M, Bentley G. A simple method of cartilage regeneration using a new polymerizing system: Ultrastructural characteristics of the repair tissue. J Mater Sci Mater Med 1994; 5: 793–797.
10.1007/BF00213137
CAS Web of Science® Google Scholar
17Downes S, Archer RS, Kayser MV, Patel MP, Braden M. The regeneration of articular cartilage using a new polymer system. J Mater Sci Mater Med 1994; 5: 88–95.
10.1007/BF00121696
CAS Web of Science® Google Scholar
18Schwartz DW, Braden M. Outlining the denture bearing area: An alternative material. J Dent 1973; 1: 179–180.
10.1016/0300-5712(73)90034-1
PubMed Google Scholar
19Braden M. Composition for use in making “bone cement.” U.S. Patent No. 4,7916,150, 1988.
Google Scholar
20Patel MP, Braden M. New methacrylates for use in orthopedic surgery. In: S Downes, M Dabestani, editors. Failure of joint replacement: A biological, surgical or mechanical problem? Middlesex, UK: The Institute of Orthopaedics; 1994.
Google Scholar
21Borchard U. Pharmacology, toxicology and clinical actions of monomeric and polymeric methylmethacrylate. In: DF Williams, editor. Systemic aspects of biocompatibility. Boca Raton, FL: CRC Press; 1989. p 107–143.
Google Scholar
22Burnett GM, Duncan GL. High conversion polymerization of vinyl systems. I. Methyl methacrylate. Die Makromol Chem 1962; 51: 154–170.
10.1002/macp.1962.020510115
CAS Web of Science® Google Scholar
23Svartling N, Pfaffi P, Tarkanen L. Methylmethacrylate blood levels in patients with femoral neck fracture. Arch Orthop Trauma Surg 1985; 104: 242–246.
10.1007/BF00450218
CAS PubMed Web of Science® Google Scholar
24Patternson BM, Healy JH, Cornell CN, Sharrock NE. Cardiac arrest during hip arthroplasty with a cemented long stem component: A report of seven cases. J Bone Joint Surg (Am) 1991; 73(2): 271–277.
10.2106/00004623-199173020-00015
PubMed Web of Science® Google Scholar
25Goodwin CJ, Braden M, Downes S, Marshall NJ. A comparison between two methacrylate cements as delivery systems for bioactive human growth hormone. J Mater Sci Mater Med 1995; 6: 590–596.
10.1007/BF00121283
CAS Web of Science® Google Scholar
26Revell P, George M, Braden M, Freeman M, Weightman B. Experimental studies of the biological response to a new bone cement. J Mater Sci Mater Med 1992; 3: 84–87.
10.1007/BF00705273
CAS Web of Science® Google Scholar
27Mir GN, Lawrence WH, Autian J. Toxicological and pharmacological actions of methacrylate monomers. I. Effects on isolated perfused rabbit heart. J Pharm Sci 1973; 62: 778–782.
10.1002/jps.2600620515
CAS PubMed Web of Science® Google Scholar
28Revell P, Braden M, Weightman B, Freeman M. Experimental studies of the biological response to a new bone cement. II. Soft tissue reactions in the rat. Clin Mater 1992; 10: 233.
10.1016/0267-6605(92)90016-M
CAS PubMed Google Scholar
29Gough J. Apoptosis of human osteoblasts cultured on polymeric biomaterials in vitro. Ph.D. thesis. University of Nottingham, UK. 1999.
Google Scholar
30Wergedal JE, Baylink DJ. Characterization of cells isolated from human bone. Proc Soc Exp Biol Med 1984; 176: 60–69.
10.3181/00379727-176-41843
CAS PubMed Web of Science® Google Scholar
31Di-Silvio L. A novel application of two biomaterials for the delivery of growth hormone and its effects on osteoblasts. Ph.D. thesis. Institute of Orthopaedics, University College London Medical School. 1995.
Google Scholar
32Martins Vale F, Castro M, Monteiro J, Couto FS, Pinto R, Rico JMGT. Acrylic bone cement induces the production of free radicals by cultured human fibroblasts. Biomaterials 1997; 18: 1133–1135.
10.1016/S0142-9612(97)00043-4
PubMed Web of Science® Google Scholar
33Ciapetti G, Granchi E, Cenni L, Cavedagna D, Pizzoferrato A. Cytotoxic effect of bone cements in HL-60 cells: Distinction between apoptosis and necrosis. J Biomed Mater Res 2000; 52: 338–345.
10.1002/1097-4636(200011)52:2<338::AID-JBM13>3.0.CO;2-L
CAS PubMed Web of Science® Google Scholar
34Gentil B, Paugam C, Wolf C, Lienhart A, Augereau B. Methylmethacrylate plasma levels during total hip arthroplasty. Clin Orthop Rel Res 1993; 287: 112–116.
PubMed Web of Science® Google Scholar
35Burg KJL, Porter S, Kellam JF. Biomaterial developments for bone tissue engineering. Biomaterials 2000; 21(23): 2347–2359.
10.1016/S0142-9612(00)00102-2
CAS PubMed Web of Science® Google Scholar
36Zumstein P, Stiles CD. Molecular cloning of gene sequences that are regulated by insulin-like growth factor-1. J Biol Chem 1987; 262: 11252–11260.
CAS PubMed Web of Science® Google Scholar
37Hill PA, Tumber A, Meikle MC. Multiple extracellular signals promote osteoblast survival and apoptosis. Endocrinology 1997; 138(9): 3849–3858.
CAS PubMed Web of Science® Google Scholar

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Volume57, Issue4

15 December 2001

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