ORIGINAL ARTICLE
Nerolidol assists Cisplatin to induce early apoptosis in human laryngeal carcinoma Hep 2 cells through ROS and mitochondrial-mediated pathway: An in vitro and in silico view
Vaitheeswari Balakrishnan,
Sindhu Ganapathy,
Vinothkumar Veerasamy,
Ramachandhiran Duraisamy,
Saranya Jawaharlal,
Vennila Lakshmanan,
Vaitheeswari Balakrishnan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Search for more papers by this authorCorresponding Author
Sindhu Ganapathy
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Department of Biochemistry, Government Arts College (Autonomous), Kumbakonam, India
Correspondence
Sindhu Ganapathy, Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608 002, Tamilnadu, India.
Email: [email protected]
Search for more papers by this authorVinothkumar Veerasamy
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Search for more papers by this authorRamachandhiran Duraisamy
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Search for more papers by this authorSaranya Jawaharlal
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Search for more papers by this authorVennila Lakshmanan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Search for more papers by this authorVaitheeswari Balakrishnan,
Sindhu Ganapathy,
Vinothkumar Veerasamy,
Ramachandhiran Duraisamy,
Saranya Jawaharlal,
Vennila Lakshmanan,
Vaitheeswari Balakrishnan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Search for more papers by this authorCorresponding Author
Sindhu Ganapathy
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Department of Biochemistry, Government Arts College (Autonomous), Kumbakonam, India
Correspondence
Sindhu Ganapathy, Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar 608 002, Tamilnadu, India.
Email: [email protected]
Search for more papers by this authorVinothkumar Veerasamy
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Search for more papers by this authorRamachandhiran Duraisamy
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Search for more papers by this authorSaranya Jawaharlal
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Search for more papers by this authorVennila Lakshmanan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, India
Search for more papers by this authorAbstract
The objective of this study was to examine Nerolidol (NER) and Cisplatin (CIS) performed against human laryngeal carcinoma (Hep 2) cells. We evaluated the effect of NER, CIS, and NER + CIS on cell viability, cell migration, oxidative stress, mitochondrial membrane depolarization, nuclear condensation, apoptotic induction, and DNA damage in Hep 2 cells. We used the MTT assay to assess the cytotoxicity effect of NER and CIS on Hep 2 cells in terms of morphological alterations. Present results demonstrated that IC50 values of NER and CIS have potential cytotoxicity against Hep 2 cells. NER effectively inhibited cell viability, increased reactive oxygen species generation, apoptotic induction, and DNA damage in Hep 2 cells. In addition, the docking study evaluated the structural binding interaction of NER with PI3K/Akt and PCNA protein. Furthermore, NER with PI3K/Akt, PCNA has a higher crucial score and affinity. Present results infer that NER could be used to target signaling molecules in anticancer studies.
Practical applications
Nerolidol is a dietary phytochemical with high biological activity that can find in a variety of plants. Many researchers focused on Nerolidol to treat various diseases including cancer. However, there is no studies exist on laryngeal cancer. This study uses Nerolidol and Cisplatin to generate oxidative stress and stimulate apoptosis and DNA damage in human laryngeal cancer cells. Based on present findings, Nerolidol could be a choice of anticancer medication, either alone or in combination against oral squamous cell carcinomas in both in vitro and in vivo experimental systems.
CONFLICT OF INTEREST
The authors declare that they have no conflict of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data supporting this study's findings are available on request from the corresponding author.
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