Gypenosides (GPs) alleviates hypoxia-induced injury in PC12 cells and enhances tolerance to anoxia in C57BL/6 mice
Cheng-Bo Wang
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
Search for more papers by this authorMing Zhao
Beijing Institute of Basic Medical Sciences, Beijing, China
Search for more papers by this authorJun Wang
Yantai Zhongke Research Institute of Advanced Materials and Green Chemical Engineering, Yantai, China
Search for more papers by this authorJiao-Tai Shi
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China
Search for more papers by this authorWei-Feng Wang
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
Search for more papers by this authorYing Zhang
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
University of Chinese Academy of Sciences, Beijing, China
Search for more papers by this authorXian-Hua Meng
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
Yantai Zhongke Research Institute of Advanced Materials and Green Chemical Engineering, Yantai, China
Search for more papers by this authorChun-Yan Sang
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
Search for more papers by this authorCorresponding Author
Ling-Ling Zhu
Beijing Institute of Basic Medical Sciences, Beijing, China
Correspondence
Jun-Li Yang, CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, China.
Email: [email protected]
Ling-Ling Zhu, Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jun-Li Yang
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
Correspondence
Jun-Li Yang, CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, China.
Email: [email protected]
Ling-Ling Zhu, Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
Email: [email protected]
Search for more papers by this authorCheng-Bo Wang
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
Search for more papers by this authorMing Zhao
Beijing Institute of Basic Medical Sciences, Beijing, China
Search for more papers by this authorJun Wang
Yantai Zhongke Research Institute of Advanced Materials and Green Chemical Engineering, Yantai, China
Search for more papers by this authorJiao-Tai Shi
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, China
Search for more papers by this authorWei-Feng Wang
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
Search for more papers by this authorYing Zhang
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
University of Chinese Academy of Sciences, Beijing, China
Search for more papers by this authorXian-Hua Meng
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
Yantai Zhongke Research Institute of Advanced Materials and Green Chemical Engineering, Yantai, China
Search for more papers by this authorChun-Yan Sang
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
Search for more papers by this authorCorresponding Author
Ling-Ling Zhu
Beijing Institute of Basic Medical Sciences, Beijing, China
Correspondence
Jun-Li Yang, CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, China.
Email: [email protected]
Ling-Ling Zhu, Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Jun-Li Yang
CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China
Correspondence
Jun-Li Yang, CAS Key Laboratory of Chemistry of Northwestern Plant Resources, Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou 730000, China.
Email: [email protected]
Ling-Ling Zhu, Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
Email: [email protected]
Search for more papers by this authorCheng-Bo Wang and Ming Zhao contributed equally to this work.
Abstract
Oxygen is a necessary substance for life activities, but reduced oxygen utilization due to high altitude exposure and respiratory dysfunction diseases could lead to pathological changes in the organisms. Herein gypenosides, the active ingredients in the food and medicine resource plant Gynostemma pentaphyllum (Thunb.) Makino were found to alleviate hypoxia-induced injury in PC12 cells. Moreover, hypoxia induced an increase in Ca2+ and reactive oxygen species content, and such patterns were both significantly reduced by gypenosides treatment. At the same time, gypenosides significantly blocked the decrease of both NO content and mitochondrial membrane potential caused by hypoxia. Furthermore, gypenosides gavage treatment significantly prolonged the survival time of C57BL/6 mice in confinement up to 24.3% and enhanced the locomotor ability of mice. Therefore, gypenosides have good neuroprotective effects and hypoxia tolerance activity and have the prospect of being developed as a preventive and therapeutic drug for hypoxia-related diseases.
Practical applications
Gypenosides can enhance tolerance of cells and mice to hypoxia and have the potential to be developed into hypoxia-resistant health food and drugs.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
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| jfbc14448-sup-0001-FigureS1.docxWord 2007 document , 23 KB |
Figure S1 |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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