Renoprotective and hepatoprotective activity of Lepidium draba L. extracts on oxymetholone-induced oxidative stress in rat
Jinge Feng
Department of Hepatobiliary Surgery, Xian Yang Central Hospital, Xianyang City, China
Search for more papers by this authorHongbo Gao
Department of Gastroenterology, People's Hospital of Zhangqiu, Jinan, China
Search for more papers by this authorLin Yang
Department of Hepatobiliary Surgery, Xian Yang Central Hospital, Xianyang City, China
Search for more papers by this authorCorresponding Author
Yu Xie
Department of Hepatobiliary Surgery, PLA Rocket Force Characteristic Medical Center, Beijing, China
Correspondence
Yu Xie, Department of Hepatobiliary Surgery, PLA Rocket Force Characteristic Medical Center, Beijing 100088, China.
Email: [email protected]
Search for more papers by this authorAyman E. El-Kenawy
Pathology Department, College of Medicine, Taif University, Taif, Saudi Arabia
Search for more papers by this authorAttalla F. El-kott
Biology Department, College of Science, King Khalid University, Abha, Saudi Arabia
Zoology Department, College of Science, Damanhour University, Damanhour, Egypt
Search for more papers by this authorJinge Feng
Department of Hepatobiliary Surgery, Xian Yang Central Hospital, Xianyang City, China
Search for more papers by this authorHongbo Gao
Department of Gastroenterology, People's Hospital of Zhangqiu, Jinan, China
Search for more papers by this authorLin Yang
Department of Hepatobiliary Surgery, Xian Yang Central Hospital, Xianyang City, China
Search for more papers by this authorCorresponding Author
Yu Xie
Department of Hepatobiliary Surgery, PLA Rocket Force Characteristic Medical Center, Beijing, China
Correspondence
Yu Xie, Department of Hepatobiliary Surgery, PLA Rocket Force Characteristic Medical Center, Beijing 100088, China.
Email: [email protected]
Search for more papers by this authorAyman E. El-Kenawy
Pathology Department, College of Medicine, Taif University, Taif, Saudi Arabia
Search for more papers by this authorAttalla F. El-kott
Biology Department, College of Science, King Khalid University, Abha, Saudi Arabia
Zoology Department, College of Science, Damanhour University, Damanhour, Egypt
Search for more papers by this authorAbstract
Misuse and abuse of anabolic androgenic steroids (AAS) such as oxymetholone (OM) cause side effects such as male infertility, cardiovascular disorders, musculoskeletal, and hepato-renal dysfunctions in athletes. The aim of this study was to evaluate the protective effects of Lepidium draba L. (L. draba) extract on OM-induced hepato-renal toxicity. Thirty adult male Wistar rats into six groups (n = 5) were randomly divided as follows: control (normal saline), OM (5 mg/kg/day), L. draba-treated (100, 200, and 400 mg/kg/d) plus 5 mg/kg/day OM, and L. draba (400 mg/kg/d) groups. Normal saline, OM and L. draba extract were orally administered for 30 days. On day 31 of the study, hepatic and renal biochemical parameters were measured. Serum cytokines (IL-1β, IL-10, IL-6) tumor necrosis factor- α (TNF-α) and nitric oxide, levels alongside catalase, glutathione peroxidase, and superoxide dismutase activity were evaluated. Also, changes in liver and kidney histopathology were evaluated. Finally, the anti-oxidant properties of the extract were determined. The results of this study showed that in the groups treated with the L. draba extract, hepatic-renal biochemical parameters improved and also the level of nitric oxide and inflammatory cytokines decreased and the activity of anti-oxidant enzymes increased compared with the OM group. These findings revealed that L. draba, due to its high anti-oxidant properties and high content of polyphenols (especially flavonoids), can improve OM-induced hepato-renal oxidative damages.
Practical applications
L. draba due to its remarkable anti-oxidant and anti-inflammatory properties can protects the kidney and liver injuries against oxymetholone. These features are attributed to the presence of phenolic and flavonoid components. This fidings would be helpful to desgin new therapeutic agents for treating and preventing liver/kidney injuries.
CONFLICT OF INTEREST
The authors declare no conflicts of interest.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available on request from the corresponding author.
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