Effects of blueberry polyphenolic extract on vascular remodeling in spontaneously hypertensive rats
Sijo Joseph Thandapilly
Agriculture and Agri-Food Canada, Winnipeg, Canada
Contribution: Data curation, Formal analysis, Writing - original draft
Search for more papers by this authorXavier Louis
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
Contribution: Data curation, Formal analysis, Writing - review & editing
Search for more papers by this authorWilhelmina Kalt
Agriculture and Agri-Food Canada, Kentville, Canada
Contribution: Conceptualization, Funding acquisition, Resources, Supervision, Writing - review & editing
Search for more papers by this authorPema Raj
Agriculture and Agri-Food Canada, Winnipeg, Canada
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
Contribution: Data curation, Formal analysis, Writing - review & editing
Search for more papers by this authorJillian L. Stobart
Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Canada
Contribution: Data curation, Formal analysis, Writing - review & editing
Search for more papers by this authorBasma M. Aloud
Agriculture and Agri-Food Canada, Winnipeg, Canada
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
Contribution: Data curation, Writing - review & editing
Search for more papers by this authorMelinda Vinqvist-Tymchuk
Agriculture and Agri-Food Canada, Kentville, Canada
Contribution: Data curation
Search for more papers by this authorLiping Yu
Agriculture and Agri-Food Canada, Winnipeg, Canada
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
Contribution: Data curation, Formal analysis
Search for more papers by this authorJacques Kaminski
Laboratory of Biochemistry of Metabolism and Nutrition, University of Burgundy, Dijon, France
Contribution: Data curation, Formal analysis, Visualization, Writing - original draft
Search for more papers by this authorNorbert Latruffe
Laboratory of Biochemistry of Metabolism and Nutrition, University of Burgundy, Dijon, France
Contribution: Writing - review & editing
Search for more papers by this authorChristopher M. Anderson
Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Canada
Search for more papers by this authorHope D. Anderson
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
College of Pharmacy, University of Manitoba, Winnipeg, Canada
Contribution: Formal analysis, Supervision, Validation, Writing - review & editing
Search for more papers by this authorCorresponding Author
Thomas Netticadan
Agriculture and Agri-Food Canada, Winnipeg, Canada
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, Canada
Correspondence
Thomas Netticadan, Canadian Centre for Agri-Food Research in Health and Medicine, 351 Tache Avenue, Winnipeg, MB R2H 2A6, Canada.
Email: [email protected]
Contribution: Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Validation, Writing - review & editing
Search for more papers by this authorSijo Joseph Thandapilly
Agriculture and Agri-Food Canada, Winnipeg, Canada
Contribution: Data curation, Formal analysis, Writing - original draft
Search for more papers by this authorXavier Louis
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
Contribution: Data curation, Formal analysis, Writing - review & editing
Search for more papers by this authorWilhelmina Kalt
Agriculture and Agri-Food Canada, Kentville, Canada
Contribution: Conceptualization, Funding acquisition, Resources, Supervision, Writing - review & editing
Search for more papers by this authorPema Raj
Agriculture and Agri-Food Canada, Winnipeg, Canada
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
Contribution: Data curation, Formal analysis, Writing - review & editing
Search for more papers by this authorJillian L. Stobart
Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Canada
Contribution: Data curation, Formal analysis, Writing - review & editing
Search for more papers by this authorBasma M. Aloud
Agriculture and Agri-Food Canada, Winnipeg, Canada
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
Contribution: Data curation, Writing - review & editing
Search for more papers by this authorMelinda Vinqvist-Tymchuk
Agriculture and Agri-Food Canada, Kentville, Canada
Contribution: Data curation
Search for more papers by this authorLiping Yu
Agriculture and Agri-Food Canada, Winnipeg, Canada
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
Contribution: Data curation, Formal analysis
Search for more papers by this authorJacques Kaminski
Laboratory of Biochemistry of Metabolism and Nutrition, University of Burgundy, Dijon, France
Contribution: Data curation, Formal analysis, Visualization, Writing - original draft
Search for more papers by this authorNorbert Latruffe
Laboratory of Biochemistry of Metabolism and Nutrition, University of Burgundy, Dijon, France
Contribution: Writing - review & editing
Search for more papers by this authorChristopher M. Anderson
Department of Pharmacology and Therapeutics, University of Manitoba, Winnipeg, Canada
Search for more papers by this authorHope D. Anderson
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
College of Pharmacy, University of Manitoba, Winnipeg, Canada
Contribution: Formal analysis, Supervision, Validation, Writing - review & editing
Search for more papers by this authorCorresponding Author
Thomas Netticadan
Agriculture and Agri-Food Canada, Winnipeg, Canada
Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada
Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, Canada
Correspondence
Thomas Netticadan, Canadian Centre for Agri-Food Research in Health and Medicine, 351 Tache Avenue, Winnipeg, MB R2H 2A6, Canada.
Email: [email protected]
Contribution: Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Validation, Writing - review & editing
Search for more papers by this authorSijo Joseph Thandapilly and Xavier Louis contributed equally to this work.
Abstract
Blueberry is considered a functional food due to various beneficial health effects associated with its consumption. Therefore, we examined the cardiovascular benefits of a blueberry polyphenolic extract in spontaneously hypertensive rats (SHR). Male SHR and Wistar–Kyoto (WKY) rats were administered with blueberry polyphenolic extract for 15 weeks. SHR showed significant augmented media-to-lumen ratio compared to WKY rats and blueberry polyphenolic extract significantly improved media-to-lumen ratio. SHR also had high blood pressure (BP), cardiac remodeling, and diastolic dysfunction and treatment did not affect BP or cardiac structure and function. SHR showed significantly increased the levels of malondialdehyde (MDA) and blueberry polyphenolic extract did not lower MDA. The levels of interleukin 6 and nitrate/nitrite ratio were unaltered in SHR. SHR showed a significant increase in the pro-apoptotic marker, Bax. Blueberry polyphenolic extract significantly lowered Bax. Our study shows that blueberry polyphenolic extract is beneficial in preventing vascular remodeling and cardiac apoptosis.
Practical applications
Similar to many other berries, blueberries are repertoire of many phytochemicals including polyphenols. Along with its considerably well-established role as a sought after berry, blueberries have been at the forefront of approaches to hharnessing health benefits from plant food sources. Several studies have attempted to unravel the role of blueberry and their major phytochemicals in reducing the risk of cardiovascular diseases and reported their beneficial effects. Our pre-clinical study found that blueberry polyphenolic extract can reduce vascular remodeling in the setting of hypertension. This new finding further suggests the potential of blueberry-based phytochemicals. Further exploration of blueberries and their phytochemicals and positive outcomes from such studies can lead to substantial benefits for consumers and economy as a whole.
CONFLICT OF INTEREST
The authors declare no conflict of interests.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
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Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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