Volume 46, Issue 3 e13866

SPECIAL ISSUE ORIGINAL ARTICLE

Taurochenodeoxycholic acid inhibits the proliferation and invasion of gastric cancer and induces its apoptosis

Dongwei Zhang,

Dongwei Zhang

Department of Oncology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

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Yongfu Zhu,

Yongfu Zhu

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

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Ya Su,

Ya Su

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - original draft

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Minghui Yu,

Minghui Yu

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

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Xiaozhou Xu,

Xiaozhou Xu

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

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Chunhua Wang,

Chunhua Wang

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

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Shaohu Zhang,

Shaohu Zhang

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

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Liming Xia,

Corresponding Author

Liming Xia

[email protected]

orcid.org/0000-0001-8510-632X

Department of Oncology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Correspondence

Liming Xia, Department of Oncology, The First Affiliated Hospital of Anhui University of Chinese Medicine, 117 Meishan Road, Shushan District, Hefei 230031, Anhui Province, China.

Email: [email protected]

Contribution: Writing - review & editing

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Dongwei Zhang,

Dongwei Zhang

Department of Oncology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

Search for more papers by this author

Yongfu Zhu,

Yongfu Zhu

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

Search for more papers by this author

Ya Su,

Ya Su

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - original draft

Search for more papers by this author

Minghui Yu,

Minghui Yu

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

Search for more papers by this author

Xiaozhou Xu,

Xiaozhou Xu

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

Search for more papers by this author

Chunhua Wang,

Chunhua Wang

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

Search for more papers by this author

Shaohu Zhang,

Shaohu Zhang

Department of Spleen and Stomach, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Contribution: Writing - review & editing

Search for more papers by this author

Liming Xia,

Corresponding Author

Liming Xia

[email protected]

orcid.org/0000-0001-8510-632X

Department of Oncology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China

Correspondence

Liming Xia, Department of Oncology, The First Affiliated Hospital of Anhui University of Chinese Medicine, 117 Meishan Road, Shushan District, Hefei 230031, Anhui Province, China.

Email: [email protected]

Contribution: Writing - review & editing

Search for more papers by this author

First published: 18 July 2021

https://doi.org/10.1111/jfbc.13866

Citations: 6

[Corrections added on October 22, 2021, after first online publication: Liming Xia's and Dongwei Zhang's affiliation has been corrected to Department of Oncology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China. The address for correspondence has been updated accordingly.]

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Abstract

Taurochenodeoxycholic acid (TCDCA) is the principal ingredient of Compound Shougong Powder. Despite traditional Chinese medicine (TCM) research demonstrates that Compound Shougong Powder can restrict tumor growth, whether TCDCA exerts a role in suppressing cancer as the major ingredient of Compound Shougong Powder remains unknown. This study aims to clarify the regulatory mechanism of TCDCA on gastric cancer. Gastric cancer cells SGC-7901 were cultured to investigate the effects of TCDCA on proliferation and apoptosis. Furthermore, a subcutaneously implanted tumor model was established using SGC-7901 cells in BALB/C nude mice and tumor volume was measured under low and high dose treatment of TCDCA. Cell proliferation, apoptosis, and invasion were subjected to 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) assay, flow cytometry, and transwell assay. Differentially expressed genes were screened by transcriptome sequencing. Nude mouse tumorigenicity assay was initiated to identify the effect of TCDCA on both tumor volume and weight, and the expression of candidate genes screened by transcriptome sequencing was determined by real-time fluorescence quantification (qPCR) and Western blot. The experiments revealed that TCDCA could significantly inhibit the proliferation and invasion of gastric cancer cells and induce apoptosis of these cells. Meanwhile, test findings via in vivo indicated that TCDCA severely diminished the volume and weight of tumors. This study first demonstrated that TCDCA inhibited the proliferation and invasion of gastric cancer and induced apoptosis, which is expected to serve as an experimental basis for the application of TCM in tumor therapeutic options.

Practical applications

Through this study, the inhibitory effect of Taurochenodeoxycholic acid on gastric cancer can be clarified, which provides a new research basis for the application of traditional Chinese medicine (TCM) and TCM monomer in cancer. In addition, this study can further promote the research and application of Chinese traditional medicine, which has important application value and economic benefits.

CONFLICT OF INTEREST

The authors declared that they have no conflict of interest.

Open Research

DATA AVAILABILITY STATEMENT

The analyzed data sets generated during the study are available from the corresponding author on request.

Supporting Information

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Taurochenodeoxycholic acid inhibits the proliferation and invasion of gastric cancer and induces its apoptosis

Abstract

Practical applications

CONFLICT OF INTEREST

Open Research

DATA AVAILABILITY STATEMENT

Supporting Information

REFERENCES

Citing Literature

References

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Taurochenodeoxycholic acid inhibits the proliferation and invasion of gastric cancer and induces its apoptosis

Abstract

Practical applications

CONFLICT OF INTEREST

Open Research

DATA AVAILABILITY STATEMENT

Supporting Information

REFERENCES

Citing Literature

References

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