Corresponding author. S-C. Wu, Department of Food Science, College of Life Science, National Chiayi University, No. 300, Syue fu Road, Chiayi City 60004, Taiwan. TEL: +886-5-2717622; FAX: +886-5-2717590; EMAIL: [email protected]Search for more papers by this author

Ling-Fang Zhang,

Ling-Fang Zhang

Department of Food Sciences, National Chiayi University, Chiayi, 60004 Taiwan

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Siou-Ru Shen,

Siou-Ru Shen

Department of Food Sciences, National Chiayi University, Chiayi, 60004 Taiwan

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Yen-Hsing Li,

Yen-Hsing Li

Department of Basic Medical Science, Purdue University, West Lafayette, IN

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Chih-Yu Lo,

Chih-Yu Lo

Department of Food Sciences, National Chiayi University, Chiayi, 60004 Taiwan

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Bao-Hong Lee,

Bao-Hong Lee

Department of Basic Medical Science, Purdue University, West Lafayette, IN

Department of Medicinal Plant Development, Yu-Pin Tang Traditional Chinese Medicine Foundation, Kaohsiung City, 813 Taiwan

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She-Ching Wu,

Corresponding Author

She-Ching Wu

Department of Food Sciences, National Chiayi University, Chiayi, 60004 Taiwan

First published: 08 October 2015

https://doi.org/10.1111/jfbc.12203

Citations: 1

The Copyright line in this article was changed on 23 February 2016 after online publication

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Abstract

Graptopetalum paraguayense was shown to exhibit antioxidant, hepatoprotective and anti-hepatocellular carcinoma activities in a recent study. In this work, fractions of the G. paraguayense aqueous solution were extracted using n-hexane, ethyl acetate (EF) and n-butanol, and its antioxidative and anti-glycative activities were evaluated. The EF extract exhibited the highest antioxidative activity, potently inhibited the formation of advanced glycation end products (AGEs) and reduced glycation-induced protein oxidation. Additionally, the EF extract protected Hep G2 cells against AGE-induced DNA damage. Five major components were isolated and identified in the EF fraction by semi-preparative high-performance liquid chromatography, liquid chromatography-tandem mass spectroscopy and nuclear magnetic resonance: gallic acid, isoquercitrin-6-(3-hydroxy-3-methylglutarate), astragalin-6-(3-hydroxy-3-methylglutarate), isoquercitrin-2-acetyl-6-(3-hydroxy-3-methylglutarate) and astragalin-2-acetyl-6-(3-hydroxy-3-methylglutarate). G. paraguayense exhibited antioxidant and anti-glycation activities, likely because of the presence of gallic acid. The findings indicate the potential for using G. paraguayense for development of novel treatments for diabetes.

Practical Applications

Herbal treatments for hyperlipidemia are relatively cheap and locally available. The results indicated that the extracts of Graptopetalum paraguayense may be developed as functional foods for antidiabetic treatment mediated by attenuations of advanced glycation end product oxidation.

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