Characterization of immune cells and infection by HIV in human ovarian tissues
Corresponding Author
Zheng Shen
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA
Correspondence
Zheng Shen, Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.
Email: [email protected]
Search for more papers by this authorMarta Rodriguez-Garcia
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA
Search for more papers by this authorChristina Ochsenbauer
Department of Medicine and UAB Center for AIDS Research, University of Alabama, Birmingham, AL, USA
Search for more papers by this authorCharles R. Wira
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA
Search for more papers by this authorCorresponding Author
Zheng Shen
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA
Correspondence
Zheng Shen, Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.
Email: [email protected]
Search for more papers by this authorMarta Rodriguez-Garcia
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA
Search for more papers by this authorChristina Ochsenbauer
Department of Medicine and UAB Center for AIDS Research, University of Alabama, Birmingham, AL, USA
Search for more papers by this authorCharles R. Wira
Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA
Search for more papers by this authorFunding Information
Study supported by NIH grants AI102838 and AI117739 (CRW), P30 AI27767 (CO)
Abstract
Problem
New HIV infections in women are predominantly spread through sexual intercourse. Recent non-human primate studies demonstrated that simian immunodeficiency virus (SIV) deposited in the vagina infected immune cells in the ovary. Whether immune cells in the human ovary are susceptible to HIV infection is unknown.
Method of study
Immune cells were isolated from ovaries and characterized by flow cytometry. Cells were exposed to HIV for 2 hours. HIV infection was measured by flow cytometry and p24 secretion following 6 days in culture.
Results
CD4+ T cells and CD14+ cells are present in the ovary and susceptible to infection by HIV-BaL. Among the CD45+ cells present, 30% were CD3+ T cells (with similar proportions of CD4+ or CD8+ T cells), and 7%-10% were CD14+ cells. Both CD4+ T cells and CD14+ cells were productively infected and supported replication.
Conclusion
Immune cells in the ovary are potential targets for HIV infection.
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