Selenate reductase activity in Escherichia coli requires Isc iron–sulfur cluster biosynthesis genes
Corresponding Author
Nathan Yee
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Correspondence: Nathan Yee, 14 College Farm Rd, New Brunswick, NJ 08901, USA. Tel.: +1 848-932-5714; fax: +1 732-932-8644;
e-mail: [email protected]
Search for more papers by this authorJessica Choi
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Search for more papers by this authorAbigail W. Porter
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Search for more papers by this authorSean Carey
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Search for more papers by this authorInes Rauschenbach
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Search for more papers by this authorArye Harel
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Search for more papers by this authorCorresponding Author
Nathan Yee
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Correspondence: Nathan Yee, 14 College Farm Rd, New Brunswick, NJ 08901, USA. Tel.: +1 848-932-5714; fax: +1 732-932-8644;
e-mail: [email protected]
Search for more papers by this authorJessica Choi
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Search for more papers by this authorAbigail W. Porter
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Search for more papers by this authorSean Carey
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Search for more papers by this authorInes Rauschenbach
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Search for more papers by this authorArye Harel
School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA
Search for more papers by this authorAbstract
The selenate reductase in Escherichia coli is a multi-subunit enzyme predicted to bind Fe–S clusters. In this study, we examined the iron–sulfur cluster biosynthesis genes that are required for selenate reductase activity. Mutants devoid of either the iscU or hscB gene in the Isc iron–sulfur cluster biosynthesis pathway lost the ability to reduce selenate. Genetic complementation by the wild-type sequences restored selenate reductase activity. The results indicate the Isc biosynthetic system plays a key role in selenate reductase Fe–S cofactor assembly and is essential for enzyme activity.
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