Study on the mechanism of targeted regulation of casein linear epitopes by three animal-derived proteases under the optimal antigen inhibition rate conditions
Qi Sun
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Qi Sun and Guangqing Mu contributed equally to this paper.
Contribution: Conceptualization, Methodology, Investigation, Data curation, Formal analysis, Writing - original draft, Validation, Visualization, Software, Writing - review & editing
Search for more papers by this authorGuangqing Mu
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Qi Sun and Guangqing Mu contributed equally to this paper.
Contribution: Conceptualization, Funding acquisition, Supervision, Resources, Project administration, Writing - review & editing
Search for more papers by this authorPeng Sun
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Contribution: Investigation, Validation, Methodology
Search for more papers by this authorXutong Ma
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Contribution: Validation, Investigation
Search for more papers by this authorQing Zhao
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Contribution: Investigation, Data curation, Formal analysis
Search for more papers by this authorXiaomeng Wu
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Contribution: Supervision, Project administration, Resources
Search for more papers by this authorShujuan Jiang
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Contribution: Supervision, Resources, Project administration
Search for more papers by this authorCorresponding Author
Fanhua Kong
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Author for correspondence. E-mail: [email protected]
Contribution: Conceptualization, Methodology, Validation, Writing - review & editing, Writing - original draft, Resources, Project administration, Investigation, Supervision, Software
Search for more papers by this authorQi Sun
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Qi Sun and Guangqing Mu contributed equally to this paper.
Contribution: Conceptualization, Methodology, Investigation, Data curation, Formal analysis, Writing - original draft, Validation, Visualization, Software, Writing - review & editing
Search for more papers by this authorGuangqing Mu
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Qi Sun and Guangqing Mu contributed equally to this paper.
Contribution: Conceptualization, Funding acquisition, Supervision, Resources, Project administration, Writing - review & editing
Search for more papers by this authorPeng Sun
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Contribution: Investigation, Validation, Methodology
Search for more papers by this authorXutong Ma
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Contribution: Validation, Investigation
Search for more papers by this authorQing Zhao
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Contribution: Investigation, Data curation, Formal analysis
Search for more papers by this authorXiaomeng Wu
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Contribution: Supervision, Project administration, Resources
Search for more papers by this authorShujuan Jiang
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Contribution: Supervision, Resources, Project administration
Search for more papers by this authorCorresponding Author
Fanhua Kong
School of Food Science and Technology, Dalian Polytechnic University, Dalian, 116000 China
Author for correspondence. E-mail: [email protected]
Contribution: Conceptualization, Methodology, Validation, Writing - review & editing, Writing - original draft, Resources, Project administration, Investigation, Supervision, Software
Search for more papers by this authorAbstract
Casein (CN) is the major family of proteins in milk and can cause allergic reactions in some infants and children. This study investigated the regulatory mechanisms of three animal-derived proteases on allergenic epitopes. The results showed that all three proteases effectively reduced the antigenicity of CN. After hydrolysis, the secondary structure of CN underwent a conformational transition from α-helix to β-turn. Based on peptidomics analysis, the allergenic epitopes of αs1-CN at positions 91–110, 131–151, 166–180 and 188–201 were effectively cleaved by pepsin, and the allergenic epitopes of β-CN at positions 60–69, 139–158 and 182–194 were effectively cleaved by pancreatic elastase.
Open Research
DATA AVAILABILITY STATEMENT
Research data are not shared.
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