Selection of robust starters from household dahi and assessment of their sensitivity to bacteriophage isolated from dairy effluents
Corresponding Author
Archana Chandran
Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi (PO), 673576 Wayanad, Kerala, India
Author for correspondence. E-mail: [email protected]
Contribution: Conceptualization, Formal analysis, Investigation, Methodology, Writing - original draft, Writing - review & editing
Search for more papers by this authorMurugadas Vaiyapuri
Central Institute of Fisheries Technology, C.I.F.T Junction, Matsyapuri, Willingdon Island, 682029 Kochi, Kerala, India
Contribution: Conceptualization, Formal analysis, Resources, Supervision, Writing - review & editing
Search for more papers by this authorRamachandran Latha Rathish
Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi (PO), 673576 Wayanad, Kerala, India
Contribution: Methodology, Visualization, Writing - review & editing
Search for more papers by this authorLijo John
Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi (PO), 673576 Wayanad, Kerala, India
Contribution: Formal analysis, Resources
Search for more papers by this authorJennifer Mahony
School of Microbiolgy and APC Microbiome Ireland, University College Cork, Western Road, T12 YT20 Cork, Ireland
Contribution: Resources, Supervision, Validation, Writing - review & editing
Search for more papers by this authorBeena Athrayil Kalathil
Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi (PO), 673576 Wayanad, Kerala, India
Contribution: Project administration, Supervision
Search for more papers by this authorCorresponding Author
Archana Chandran
Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi (PO), 673576 Wayanad, Kerala, India
Author for correspondence. E-mail: [email protected]
Contribution: Conceptualization, Formal analysis, Investigation, Methodology, Writing - original draft, Writing - review & editing
Search for more papers by this authorMurugadas Vaiyapuri
Central Institute of Fisheries Technology, C.I.F.T Junction, Matsyapuri, Willingdon Island, 682029 Kochi, Kerala, India
Contribution: Conceptualization, Formal analysis, Resources, Supervision, Writing - review & editing
Search for more papers by this authorRamachandran Latha Rathish
Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi (PO), 673576 Wayanad, Kerala, India
Contribution: Methodology, Visualization, Writing - review & editing
Search for more papers by this authorLijo John
Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi (PO), 673576 Wayanad, Kerala, India
Contribution: Formal analysis, Resources
Search for more papers by this authorJennifer Mahony
School of Microbiolgy and APC Microbiome Ireland, University College Cork, Western Road, T12 YT20 Cork, Ireland
Contribution: Resources, Supervision, Validation, Writing - review & editing
Search for more papers by this authorBeena Athrayil Kalathil
Kerala Veterinary and Animal Sciences University, Pookode, Lakkidi (PO), 673576 Wayanad, Kerala, India
Contribution: Project administration, Supervision
Search for more papers by this authorAbstract
Identifying the predominant strains of lactic acid bacteria (LAB) in dahi that are resistant to bacteriophages is essential for developing robust starter cultures. Dahi samples from two agroecological zones of Kerala were collected for isolation of LAB. Limosilactobacillus fermentum emerged as the dominant starter culture species based on sequence-based identification and technological property characterisation. Dairy effluents were screened for phages targeting L. fermentum, and 10 of 11 strains of L. fermentum showed resistance to one lytic phage isolated as part of this study. The strain ADMT 15 showed sensitivity to the phage and on subsequent genome sequencing the phage revealed its close relationship to members of the Herelleviridae phage family.
CONFLICT OF INTEREST STATEMENT
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| idt13083-sup-0001-AppendixS1.docxWord 2007 document , 41.2 KB |
Table S1 List of identified lactic acid bacteria from household dahi. Table S2 Annotation of LPhi1ADMT15 partial genome with predicted functions based on BLASTp and HHPred analysis. Figure S1 The sensitivity of LAB against phage in the effluent sample using spot test for phage LPhi1ADMT15 on strain ADMT-15 showing clear zone of lysis. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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