The Rough Morphotype of Mycobacterium abscessus Enhances Its Virulence Through ROS/p65/NLRP3/GSDMD-Mediated Macrophage Pyroptosis
Jingren Li
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorJuan Li
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorAnqi Li
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorZhili Tan
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorJunsheng Fan
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorSiyuan He
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorQi Guo
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorCorresponding Author
Liyun Xu
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Correspondence:
Liyun Xu ([email protected])
Haiqing Chu ([email protected])
Search for more papers by this authorCorresponding Author
Haiqing Chu
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Correspondence:
Liyun Xu ([email protected])
Haiqing Chu ([email protected])
Search for more papers by this authorJingren Li
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorJuan Li
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorAnqi Li
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorZhili Tan
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorJunsheng Fan
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorSiyuan He
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorQi Guo
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Search for more papers by this authorCorresponding Author
Liyun Xu
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Correspondence:
Liyun Xu ([email protected])
Haiqing Chu ([email protected])
Search for more papers by this authorCorresponding Author
Haiqing Chu
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
School of Medicine, Tongji University, Shanghai, China
Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai, China
Correspondence:
Liyun Xu ([email protected])
Haiqing Chu ([email protected])
Search for more papers by this authorABSTRACT
The rough morphotype of Mycobacterium abscessus exhibits significantly higher virulence compared to the smooth morphotype, yet the underlying molecular mechanisms remain incompletely understood. Pyroptosis in macrophages plays a pivotal role in lung tissue damage; however, its specific involvement in Mycobacterium abscessus infection remains to be fully clarified. In this study, we identified that the rough morphotype of Mycobacterium abscessus upregulates the ROS/p65/NLRP3/GSDMD signalling pathway, thereby mediating pyroptosis in THP-1-derived macrophages. This heightened ability to induce macrophage pyroptosis is attributed to the bacterium's capacity to sustain intracellular viability and proliferation. These findings offer valuable insights into the virulence mechanisms of Mycobacterium abscessus and provide a foundation for future therapeutic interventions.
Conflicts of Interest
The authors declare no conflicts of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
| Filename | Description |
|---|---|
| cep70034-sup-0001-FigureS1.tifTIFF image, 80.9 KB |
Figure S1. The total CFU count of M. abscessus within macrophages at 48 h post-infection. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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