Ultrasensitive Bi-Mode Lateral-Flow Assay via UCNPs-Based Host-Guest Assembly of Fluorescent-Colorimetric Nanoparticles
Zhujun Ai
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorHuan Cai
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorChangjin Liu
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Department of Laboratory Medicine, The Fifth People's Hospital of Chongqing, Chongqing, 400062 P. R. China
Search for more papers by this authorYan Zhao
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorQing Fu
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorNingke Fan
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorYujian Li
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorSiqiao Li
Department of Forensic Medicine, School of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorSong Zhou
Department of Forensic Medicine, School of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorChunyang Li
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorCorresponding Author
Juan Li
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Western Institute of Digital-Intelligent Medicine, Chongqing National Biological Industry Base, Chongqing, 401329 P. R. China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Shijia Ding
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Western Institute of Digital-Intelligent Medicine, Chongqing National Biological Industry Base, Chongqing, 401329 P. R. China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Rui Chen
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Western Institute of Digital-Intelligent Medicine, Chongqing National Biological Industry Base, Chongqing, 401329 P. R. China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorZhujun Ai
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorHuan Cai
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorChangjin Liu
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Department of Laboratory Medicine, The Fifth People's Hospital of Chongqing, Chongqing, 400062 P. R. China
Search for more papers by this authorYan Zhao
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorQing Fu
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorNingke Fan
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorYujian Li
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorSiqiao Li
Department of Forensic Medicine, School of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorSong Zhou
Department of Forensic Medicine, School of Basic Medical Sciences, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorChunyang Li
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Search for more papers by this authorCorresponding Author
Juan Li
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Western Institute of Digital-Intelligent Medicine, Chongqing National Biological Industry Base, Chongqing, 401329 P. R. China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Shijia Ding
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Western Institute of Digital-Intelligent Medicine, Chongqing National Biological Industry Base, Chongqing, 401329 P. R. China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorCorresponding Author
Rui Chen
Key Laboratory of Clinical Laboratory Diagnostics (Ministry of Education), College of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016 P. R. China
Western Institute of Digital-Intelligent Medicine, Chongqing National Biological Industry Base, Chongqing, 401329 P. R. China
E-mail: [email protected]; [email protected]; [email protected]
Search for more papers by this authorAbstract
Fluorescent-colorimetric nanoparticles (FCNPs) attract considerable interest as an emerging dual-signal reporter for on-site qualitative/quantitative point-of-care testing. However, the suboptimal signaling components and self-assembled structure lacking physical isolation in traditional FCNPs result in low fluorescence brightness, poor stability, and strong internal filtration effect (IFE), which severely limits their wide application in lateral flow assay (LFA). Here, ordered self-assembly for hydrophobic upconversion nanoparticles (UCNPs) is developed using 3D porous space magnetic dendritic mesoporous silica (MS), stepwise surface silanization, and polydopamine (PDA) flexible scaffold modification to fabricate MS@UCNPs@PDA (MSUD). With rational design, MSUD improves stability and luminescence intensity (131 times higher than quantum dot-based fluorophores), and also eliminates IFE and fluorescence background interference on LFA strips. The detection limits of MSUD-labeled LFA for qualitative and quantitative detection of methamphetamine by naked eye-based colorimetric and smartphone-based fluorescence strategy are 1.047 × 104 pg mL−1 and 47.25 pg mL−1, ≈10- and 2116- times lower than that of gold nanoparticles-LFA, respectively. The practicality of the MSUD-based LFA is validated in 83 urine/hair forensic samples, with the quantitative determination results in good agreement with the liquid chromatography-mass spectrometer data. This work presents an innovative strategy for constructing FCNPs, facilitating their progressive development and widespread applications.
Conflict of Interest
The authors declare no conflict of interest.
Open Research
Data Availability Statement
The data that support the findings of this study are available from the corresponding author upon reasonable request.
Supporting Information
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| smll202410947-sup-0001-SuppMat.docx23.4 MB | Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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