A Self-Assembled Protein Nanotube with High Aspect Ratio
Frederico F. Miranda
Protein Design Laboratory Yokohama City University 1-7-29 Suehiro-cho Tsurumi-ku
Search for more papers by this authorKenji Iwasaki
Laboratory of Protein Synthesis and Expression Institute for Protein Research, Osaka University Suita, Osaka, Japan and CREST Japan Science and Technology Agency 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012 (Japan)
Search for more papers by this authorSatoko Akashi
Structural Biology Laboratory Yokohama City University 1-7-29 Suehiro-cho Tsurumi-ku Yokohama, Kanagawa, 230-0045 (Japan)
Search for more papers by this authorKoji Sumitomo
NTT Basic Research Laboratories 3-1 Morinosato-Wakamiya, Atsugi Kanagawa 243-0198 (Japan)
Search for more papers by this authorMime Kobayashi
Graduate School of Materials Science Nara Institute of Science and Technology 8916-5 Takayama, Ikoma, Nara 630-0192 (Japan)
Search for more papers by this authorIchiro Yamashita
CREST, Japan Science and Technology Agency 4-1-8 Honcho, Kawaguchi Saitama, 332-0012 (Japan)
Graduate School of Materials Science Nara Institute of Science and Technology 8916-5 Takayama, Ikoma, Nara 630-0192 (Japan)
Search for more papers by this authorCorresponding Author
Jeremy R. H. Tame
Protein Design Laboratory Yokohama City University 1-7-29 Suehiro-cho Tsurumi-ku
Jeremy R. H. Tame, Protein Design Laboratory Yokohama City University 1-7-29 Suehiro-cho Tsurumi-ku.
Jonathan G. Heddle, Global Edge Institute Tokyo Institute of Technology 4259 S2-17, Nagatsuda, Midori-ku Yokohama, Kanagawa 226-8501 (Japan).
Search for more papers by this authorCorresponding Author
Jonathan G. Heddle
Global Edge Institute Tokyo Institute of Technology 4259 S2-17, Nagatsuda, Midori-ku Yokohama, Kanagawa 226-8501 (Japan)
Jeremy R. H. Tame, Protein Design Laboratory Yokohama City University 1-7-29 Suehiro-cho Tsurumi-ku.
Jonathan G. Heddle, Global Edge Institute Tokyo Institute of Technology 4259 S2-17, Nagatsuda, Midori-ku Yokohama, Kanagawa 226-8501 (Japan).
Search for more papers by this authorFrederico F. Miranda
Protein Design Laboratory Yokohama City University 1-7-29 Suehiro-cho Tsurumi-ku
Search for more papers by this authorKenji Iwasaki
Laboratory of Protein Synthesis and Expression Institute for Protein Research, Osaka University Suita, Osaka, Japan and CREST Japan Science and Technology Agency 4-1-8 Honcho, Kawaguchi, Saitama, 332-0012 (Japan)
Search for more papers by this authorSatoko Akashi
Structural Biology Laboratory Yokohama City University 1-7-29 Suehiro-cho Tsurumi-ku Yokohama, Kanagawa, 230-0045 (Japan)
Search for more papers by this authorKoji Sumitomo
NTT Basic Research Laboratories 3-1 Morinosato-Wakamiya, Atsugi Kanagawa 243-0198 (Japan)
Search for more papers by this authorMime Kobayashi
Graduate School of Materials Science Nara Institute of Science and Technology 8916-5 Takayama, Ikoma, Nara 630-0192 (Japan)
Search for more papers by this authorIchiro Yamashita
CREST, Japan Science and Technology Agency 4-1-8 Honcho, Kawaguchi Saitama, 332-0012 (Japan)
Graduate School of Materials Science Nara Institute of Science and Technology 8916-5 Takayama, Ikoma, Nara 630-0192 (Japan)
Search for more papers by this authorCorresponding Author
Jeremy R. H. Tame
Protein Design Laboratory Yokohama City University 1-7-29 Suehiro-cho Tsurumi-ku
Jeremy R. H. Tame, Protein Design Laboratory Yokohama City University 1-7-29 Suehiro-cho Tsurumi-ku.
Jonathan G. Heddle, Global Edge Institute Tokyo Institute of Technology 4259 S2-17, Nagatsuda, Midori-ku Yokohama, Kanagawa 226-8501 (Japan).
Search for more papers by this authorCorresponding Author
Jonathan G. Heddle
Global Edge Institute Tokyo Institute of Technology 4259 S2-17, Nagatsuda, Midori-ku Yokohama, Kanagawa 226-8501 (Japan)
Jeremy R. H. Tame, Protein Design Laboratory Yokohama City University 1-7-29 Suehiro-cho Tsurumi-ku.
Jonathan G. Heddle, Global Edge Institute Tokyo Institute of Technology 4259 S2-17, Nagatsuda, Midori-ku Yokohama, Kanagawa 226-8501 (Japan).
Search for more papers by this authorAbstract
Production of a self-assembled protein nanotube achieved through engineering of the 11mer ring protein trp RNA-binding attenuation protein is described. The produced mutant protein is able to stack in solution to produce an extremely narrow, uniform nanotube apparently stabilized by a mixture of disulfide bonds and hydrophobic interactions. Assembly is reversible and the length of tube can potentially be controlled. Large quantities of hollow tubes 8.5 nm in overall diameter with lengths varying from 7 nm to over 1 µm are produced. The structure is analyzed using transmission electron microscopy, atomic force microscopy, mass spectrometry, and single-particle analysis and it is found that component rings stack in a head-to-head fashion. The internal diameter of the tube is 2.5 nm, and the amino acid residues lining the central cavity can be mutated, raising the possibility that the tube can be filled with a variety of conducting or semiconducting materials.
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