The three-dimensional structure of “Lonely Guy” from Claviceps purpurea provides insights into the phosphoribohydrolase function of Rossmann fold-containing lysine decarboxylase-like proteins
Lenka Dzurová
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, 783 71 Czech Republic
Search for more papers by this authorCorresponding Author
Federico Forneris
The Armenise-Harvard Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia, Pavia, 27100 Italy
Correspondence to: Federico Forneris, The Armenise-Harvard Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9/A, Pavia 27100, Italy. E-mail: [email protected] (or) Ivo Frébort, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc 783 71, Czech Republic. E-mail: [email protected]Search for more papers by this authorSimone Savino
The Armenise-Harvard Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia, Pavia, 27100 Italy
Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia, Pavia, 27100 Italy
Search for more papers by this authorPetr Galuszka
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, 783 71 Czech Republic
Search for more papers by this authorJosef Vrabka
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, 783 71 Czech Republic
Search for more papers by this authorCorresponding Author
Ivo Frébort
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, 783 71 Czech Republic
Correspondence to: Federico Forneris, The Armenise-Harvard Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9/A, Pavia 27100, Italy. E-mail: [email protected] (or) Ivo Frébort, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc 783 71, Czech Republic. E-mail: [email protected]Search for more papers by this authorLenka Dzurová
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, 783 71 Czech Republic
Search for more papers by this authorCorresponding Author
Federico Forneris
The Armenise-Harvard Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia, Pavia, 27100 Italy
Correspondence to: Federico Forneris, The Armenise-Harvard Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9/A, Pavia 27100, Italy. E-mail: [email protected] (or) Ivo Frébort, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc 783 71, Czech Republic. E-mail: [email protected]Search for more papers by this authorSimone Savino
The Armenise-Harvard Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia, Pavia, 27100 Italy
Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia, Pavia, 27100 Italy
Search for more papers by this authorPetr Galuszka
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, 783 71 Czech Republic
Search for more papers by this authorJosef Vrabka
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, 783 71 Czech Republic
Search for more papers by this authorCorresponding Author
Ivo Frébort
Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, 783 71 Czech Republic
Correspondence to: Federico Forneris, The Armenise-Harvard Laboratory of Structural Biology, Department of Biology and Biotechnology, University of Pavia, Via Ferrata 9/A, Pavia 27100, Italy. E-mail: [email protected] (or) Ivo Frébort, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, Olomouc 783 71, Czech Republic. E-mail: [email protected]Search for more papers by this authorThis work was performed in all institutions indicated in the author affiliation.
ABSTRACT
The recently discovered cytokinin (CK)-specific phosphoribohydrolase “Lonely Guy” (LOG) is a key enzyme of CK biosynthesis, converting inactive CK nucleotides into biologically active free bases. We have determined the crystal structures of LOG from Claviceps purpurea (cpLOG) and its complex with the enzymatic product phosphoribose. The structures reveal a dimeric arrangement of Rossmann folds, with the ligands bound to large pockets at the interface between cpLOG monomers. Structural comparisons highlight the homology of cpLOG to putative lysine decarboxylases. Extended sequence analysis enabled identification of a distinguishing LOG sequence signature. Taken together, our data suggest phosphoribohydrolase activity for several proteins of unknown function. Proteins 2015; 83:1539–1546. © 2015 Wiley Periodicals, Inc.
Supporting Information
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