Fabrication and characterization of the 3D-printed polycaprolactone/fish bone extract scaffolds for bone tissue regeneration
Seong-Yeong Heo
Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, Republic of Korea
Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea
Search for more papers by this authorSeok-Chun Ko
Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea
Search for more papers by this authorGun-Woo Oh
Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, Republic of Korea
Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea
Search for more papers by this authorNamwon Kim
Ingram School of Engineering, Texas State University, San Marcos, Texas
Search for more papers by this authorIl-Whan Choi
Department of Microbiology, Inje University College of Medicine, Busan, Republic of Korea
Search for more papers by this authorWon Sun Park
Department of Physiology, Kangwon National University School of Medicine, Chuncheon, Republic of Korea
Search for more papers by this authorCorresponding Author
Won-Kyo Jung
Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, Republic of Korea
Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea
Correspondence to: W.-K. Jung; e-mail address: e-mail: [email protected]Search for more papers by this authorSeong-Yeong Heo
Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, Republic of Korea
Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea
Search for more papers by this authorSeok-Chun Ko
Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea
Search for more papers by this authorGun-Woo Oh
Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, Republic of Korea
Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea
Search for more papers by this authorNamwon Kim
Ingram School of Engineering, Texas State University, San Marcos, Texas
Search for more papers by this authorIl-Whan Choi
Department of Microbiology, Inje University College of Medicine, Busan, Republic of Korea
Search for more papers by this authorWon Sun Park
Department of Physiology, Kangwon National University School of Medicine, Chuncheon, Republic of Korea
Search for more papers by this authorCorresponding Author
Won-Kyo Jung
Department of Biomedical Engineering, and Center for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan, Republic of Korea
Marine-Integrated Bionics Research Center, Pukyong National University, Busan, Republic of Korea
Correspondence to: W.-K. Jung; e-mail address: e-mail: [email protected]Search for more papers by this authorAbstract
Fish bone extract (FBE) containing a trioligopeptide (FBP-KSA, Lys-Ser-Ala) isolated from Johnius belengerii could induce osteogenic activities on MC3T3-E1 pre-osteoblasts in our previous study. Regarding the osteogenic effect of FBE, in the present study, we fabricated the three-dimensional (3D) interconnected polycaprolactone (PCL)/FBE scaffolds for bone tissue regeneration. After fabrication of PCL scaffolds using 3D printing, FBE was coated on the surface of PCL scaffolds by self-assembly process. In the physical characteristic and mechanical property tests, the results demonstrated that the fabricated scaffolds have the strut diameter (between 340 and 345 μm), pore size (between 470 and 480 μm), porosity (between 50% and 55%), and tensile properties (Young's modulus: 9.18–9.42 MPa; max tensile strengths 82.3–87.4 MPa) were similar to those of PCL scaffold. In the cell proliferation and osteogenic assay, the results showed that PCL/FBE scaffolds could significantly induce cell proliferation, calcium deposition, and the expression of osteogenic phenotype markers such as alkaline phosphatase, osteopontin, osteocalcin, and bone morphogenetic protein-2 in the osteoblasts. These results suggest that FBE-coated PCL scaffolds are promising materials for use in biomedical application to promote bone tissue regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 1937–1944, 2019.
Supporting Information
| Filename | Description |
|---|---|
| jbmb34286-sup-0001-FigureS1.tifTIFF image, 400.8 KB | Figure S1 Schematic diagram of purification of fish bone peptide (FBP-KSA) from fish bone extracts. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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