Fibronectin grafting to enhance skin sealing around transcutaneous titanium implant
Souhaila Ghadhab
Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, 10 rue de l'Espinay⎜, Québec, Canada
Centre de Recherche sur les Matériaux Avancés (CERMA), Département de Génie des Mines, de la Métallurgie et des Matériaux, Université Laval, Québec, Canada
Search for more papers by this authorIbrahim Bilem
Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, 10 rue de l'Espinay⎜, Québec, Canada
Search for more papers by this authorAndrée-Anne Guay-Bégin
Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, 10 rue de l'Espinay⎜, Québec, Canada
Search for more papers by this authorPascale Chevallier
Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, 10 rue de l'Espinay⎜, Québec, Canada
Centre de Recherche sur les Matériaux Avancés (CERMA), Département de Génie des Mines, de la Métallurgie et des Matériaux, Université Laval, Québec, Canada
Search for more papers by this authorFrançois A. Auger
CHU de Québec-Université Laval, LOEX, Aile-R, 1401 18ième Rue, Québec, Québec, G1J 1Z4 Canada
Search for more papers by this authorJean Ruel
Département de Génie mécanique, Université Laval, Québec, Canada
Search for more papers by this authorEmmanuel Pauthe
Biomaterials for Health Research Group, ERRMECe, Équipe de recherche sur les Relations Matrice Extracellulaire-Cellules (EA1391), Institut des matériaux I-MAT (FD4122), CY Tech, CY Cergy Paris University, Maison Internationale de la Recherche (MIR), Cergy, France
Search for more papers by this authorCorresponding Author
Gaétan Laroche
Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, 10 rue de l'Espinay⎜, Québec, Canada
Centre de Recherche sur les Matériaux Avancés (CERMA), Département de Génie des Mines, de la Métallurgie et des Matériaux, Université Laval, Québec, Canada
Correspondence
Gaétan Laroche, Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche sur les Matériaux Avancés (CERMA), Département de Génie des Mines, de la Métallurgie et des Matériaux, Université Laval, Québec, Canada.
Email: [email protected]
Search for more papers by this authorSouhaila Ghadhab
Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, 10 rue de l'Espinay⎜, Québec, Canada
Centre de Recherche sur les Matériaux Avancés (CERMA), Département de Génie des Mines, de la Métallurgie et des Matériaux, Université Laval, Québec, Canada
Search for more papers by this authorIbrahim Bilem
Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, 10 rue de l'Espinay⎜, Québec, Canada
Search for more papers by this authorAndrée-Anne Guay-Bégin
Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, 10 rue de l'Espinay⎜, Québec, Canada
Search for more papers by this authorPascale Chevallier
Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, 10 rue de l'Espinay⎜, Québec, Canada
Centre de Recherche sur les Matériaux Avancés (CERMA), Département de Génie des Mines, de la Métallurgie et des Matériaux, Université Laval, Québec, Canada
Search for more papers by this authorFrançois A. Auger
CHU de Québec-Université Laval, LOEX, Aile-R, 1401 18ième Rue, Québec, Québec, G1J 1Z4 Canada
Search for more papers by this authorJean Ruel
Département de Génie mécanique, Université Laval, Québec, Canada
Search for more papers by this authorEmmanuel Pauthe
Biomaterials for Health Research Group, ERRMECe, Équipe de recherche sur les Relations Matrice Extracellulaire-Cellules (EA1391), Institut des matériaux I-MAT (FD4122), CY Tech, CY Cergy Paris University, Maison Internationale de la Recherche (MIR), Cergy, France
Search for more papers by this authorCorresponding Author
Gaétan Laroche
Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche du Centre Hospitalier Universitaire de Québec, Hôpital St-François d'Assise, 10 rue de l'Espinay⎜, Québec, Canada
Centre de Recherche sur les Matériaux Avancés (CERMA), Département de Génie des Mines, de la Métallurgie et des Matériaux, Université Laval, Québec, Canada
Correspondence
Gaétan Laroche, Laboratoire d'Ingénierie de Surface (LIS), Centre de Recherche sur les Matériaux Avancés (CERMA), Département de Génie des Mines, de la Métallurgie et des Matériaux, Université Laval, Québec, Canada.
Email: [email protected]
Search for more papers by this authorFunding information: Natural Science and Engineering Research Council of Canada, Grant/Award Number: CRDPJ 492208-15; Natural Sciences and Engineering Research Council of Canada
Abstract
Intraosseous transcutaneous amputation prosthesis is a new approach in orthopedic implants that overcomes socket prosthesis problems. Its long-term performance requires a tight skin-implant seal to prevent infections. In this study, fibronectin (Fn), a widely used adhesion protein, was adsorbed or grafted onto titanium alloy. Fn grafting was performed using two different linking arms, dopamine/glutaric anhydride or phosphonate. The characterization of Fn-modified surfaces showed that Fn grating via phosphonate has led to the highest amount of Fn cell-binding site (RGD, arginine, glycine, and aspartate) available on the surface. Interestingly, cell culture studies revealed a strong correlation between the amount of available RGD ligands and cellular behavior, since enhanced proliferation and spreading of fibroblasts were noticed on Fn-grafted surfaces via phosphonate. In addition, an original in vitro mechanical test, inspired from the real situation, to better predict clinical outcomes after implant insertion, has been developed. Tensile test data showed that the adhesion strength of a bio-engineered dermal tissue was significantly higher around Fn-grafted surfaces via phosphonate, as compared to untreated surfaces. This study sheds light on the importance of an appropriate selection of the linking arm to tightly control the spatial conformation of biomolecules on the material surface, and consequently cell interactions at the interface tissue/implant.
Open Research
DATA AVAILABILITY STATEMENT
Data available on request from the authors.
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