Volume 5, Issue 7 pp. 550-558
Full Article

Comparison of two methods for noninvasive determination of carotenoids in human and animal skin: Raman spectroscopy versus reflection spectroscopy

Maxim E. Darvin

Corresponding Author

Maxim E. Darvin

Charité – Universitätsmedizin Berlin, Department of Dermatology, Venerology and Allergology, Center of Experimental and Cutaneous Physiology CCP, Charitéplatz 1, 10117 Berlin, Germany

Maxim E. Darvin, Phone: +49 30 450 518 208, Fax: +49 30 450 518 918

Wolfgang Koecher, Phone: +49 561 52 14 10, Fax: +49 561 52 14 111

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Carl Sandhagen

Carl Sandhagen

Opsolution Spectroscopic Systems GmbH, Goethestr. 25–27, 34119 Kassel, Germany

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Wolfgang Koecher

Corresponding Author

Wolfgang Koecher

Opsolution Spectroscopic Systems GmbH, Goethestr. 25–27, 34119 Kassel, Germany

Maxim E. Darvin, Phone: +49 30 450 518 208, Fax: +49 30 450 518 918

Wolfgang Koecher, Phone: +49 561 52 14 10, Fax: +49 561 52 14 111

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Wolfram Sterry

Wolfram Sterry

Charité – Universitätsmedizin Berlin, Department of Dermatology, Venerology and Allergology, Center of Experimental and Cutaneous Physiology CCP, Charitéplatz 1, 10117 Berlin, Germany

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Juergen Lademann

Juergen Lademann

Charité – Universitätsmedizin Berlin, Department of Dermatology, Venerology and Allergology, Center of Experimental and Cutaneous Physiology CCP, Charitéplatz 1, 10117 Berlin, Germany

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Martina C. Meinke

Martina C. Meinke

Charité – Universitätsmedizin Berlin, Department of Dermatology, Venerology and Allergology, Center of Experimental and Cutaneous Physiology CCP, Charitéplatz 1, 10117 Berlin, Germany

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First published: 23 January 2012
Citations: 46

Abstract

Based on compelling in vivo and in vitro studies on human skin, carotenoids are thought to be of great interest as powerful antioxidants acting to prevent free-radical-induced damages, including premature skin ageing and the development of skin diseases such as cancer. Among the available techniques that are suitable for noninvasive determination of carotenoids in human skin, are resonance Raman spectroscopy (RRS) and reflection spectroscopy (RS). For RS, a LED-based miniaturized spectroscopic system (MSS) was developed for noninvasive measurement of carotenoids in human skin. The optimization and subsequent calibration of the MSS was performed with the use of RRS. A strong correlation between the carotenoid concentration determined by the RS and for the RRS system was achieved for human skin in vivo (R = 0.88) and for bovine udder skin in vitro (R = 0.81). (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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