Application of Nanopore Single Molecule Detection Technology in Analysis of Xylan Dissolved in Ionic Liquid†
Wanyi Xie
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714 China
Search for more papers by this authorShaoxi Fang
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Search for more papers by this authorBohua Yin
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Changchun University of Science and Technology, Changchun, Jilin, 130022 China
Search for more papers by this authorRong Tian
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714 China
Search for more papers by this authorLiyuan Liang
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714 China
Search for more papers by this authorCorresponding Author
Shixuan He
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714 China
E-mail: [email protected]; [email protected]Search for more papers by this authorCorresponding Author
Deqiang Wang
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714 China
E-mail: [email protected]; [email protected]Search for more papers by this authorWanyi Xie
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714 China
Search for more papers by this authorShaoxi Fang
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Search for more papers by this authorBohua Yin
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Changchun University of Science and Technology, Changchun, Jilin, 130022 China
Search for more papers by this authorRong Tian
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714 China
Search for more papers by this authorLiyuan Liang
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714 China
Search for more papers by this authorCorresponding Author
Shixuan He
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714 China
E-mail: [email protected]; [email protected]Search for more papers by this authorCorresponding Author
Deqiang Wang
Chongqing Key Laboratory of Multi-scale Manufacturing Technology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing, 400714 China
Chongqing School, University of Chinese Academy of Sciences, Chongqing, 400714 China
E-mail: [email protected]; [email protected]Search for more papers by this authorDedicated to the Special Issue of Single-Molecule Measurement and Imaging.
Comprehensive Summary
Xylan is the most abundant hemicellulose in nature. As a new type of green organic solvent, ionic liquid shows good preservation ability for the functional groups of hemicellulose. In this paper, a single molecule detection technology based on glass nanopore was established to analyze xylan dissolved in ionic liquid. Arabino-xylan (AX) and beech xylan (BX) are respectively taken as the representatives of heterogeneous xylan and homogeneous xylan. Firstly, unmodified glass nanopore was used to detect the dissolved xylan in ionic liquid, and then poly(ethylene imine) (PEI) was used to modify the nanopore to change the surface charge in the nanopore and further enhance the interaction between the nanopore and the xylan molecule. It was found that before and after nanopore modification, at negative voltage and low positive voltage, AX didn't generate current blocking signal. On the contrary, BX didn't generate current blocking signal at positive voltage. This phenomenon may be due to the current disturbance driven by electrophoresis and electroosmosis of xylan molecules with weak negative charge. After statistics analysis, the current blocking signal of AX showed that the modified nanopore showed multiple peaks. It indicates that heterogeneous xylan and PEI modified nanopore had stronger interaction. The results show that the nanopore detection technology can show the structural difference of heterogenous branched chain and homogeneous straight chain based on the single characteristic current blocking signal and statistical information, providing a research basis for the structural analysis of water insoluble polysaccharides.
Supporting Information
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Appendix S1: Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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