Exosome-Coated Zeolitic Imidazolate Framework Nanoparticles for Intracellular Detection of ATP†
Wenxing Lv
Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072 China
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
‡W. L. and Z. H. contributed equally.
† Dedicate to the Special Issue of In Situ Target Biomolecule Analysis in Confined Nanospace.
Search for more papers by this authorZiwei Han
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049 China
‡W. L. and Z. H. contributed equally.
† Dedicate to the Special Issue of In Situ Target Biomolecule Analysis in Confined Nanospace.
Search for more papers by this authorYike Li
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorYanjuan Huang
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorJiashu Sun
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorCorresponding Author
Xiaoquan Lu
Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072 China
Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu, 730070 China
E-mail: [email protected]; [email protected]Search for more papers by this authorCorresponding Author
Chao Liu
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049 China
E-mail: [email protected]; [email protected]Search for more papers by this authorWenxing Lv
Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072 China
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
‡W. L. and Z. H. contributed equally.
† Dedicate to the Special Issue of In Situ Target Biomolecule Analysis in Confined Nanospace.
Search for more papers by this authorZiwei Han
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049 China
‡W. L. and Z. H. contributed equally.
† Dedicate to the Special Issue of In Situ Target Biomolecule Analysis in Confined Nanospace.
Search for more papers by this authorYike Li
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorYanjuan Huang
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorJiashu Sun
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049 China
Search for more papers by this authorCorresponding Author
Xiaoquan Lu
Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072 China
Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou, Gansu, 730070 China
E-mail: [email protected]; [email protected]Search for more papers by this authorCorresponding Author
Chao Liu
Beijing Engineering Research Center for BioNanotechnology, CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190 China
School of Future Technology, University of Chinese Academy of Sciences, Beijing, 100049 China
E-mail: [email protected]; [email protected]Search for more papers by this authorMain observation and conclusion
The intracellular delivery of metal-organic frameworks (MOFs) encapsulated with functional biomolecules represents a promising avenue in the field of biomedicine and biosensing. To improve the cellular uptake efficiency of MOFs, here we report the fabrication of cancer cell-derived exosome membrane (EM)-coated zeolitic imidazolate framework-8 (EM-ZIF-8) nanoparticles by using a microfluidic sonication device. EM-ZIF-8 nanoparticles loaded with FITC-labeled bovine serum albumin (BSA) can be taken up by cancer cells and evade phagocytosis more efficiently than their counterparts (ZIF-8 nanoparticles). Moreover, we use EM-ZIF-8 loaded with Rhodamine B (RhB) for in situ imaging of cellular adenosine triphosphate (ATP). The shield effect of ZIF-8 suppresses the fluorescence of RhB, and the presence of ATP disrupts the ZIF-8 structure based on the competitive coordination between ATP and Zn2+, leading to the restoration of RhB fluorescence. This method allows accurate detection of the fluctuation of ATP in A549 cells induced by Ca2+ or 2-DDG treatment. The devised biomimetic EM-ZIF-8 nanoparticles thus provide an efficient platform for intracellular drug delivery and ATP sensing.
Supporting Information
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Appendix S1: Supporting Information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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