Single Particle-Based Confocal Laser Scanning Microscopy for Visual Detection of Copper Ions in Confined Space†
Ke Wang
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, 710062 China
Search for more papers by this authorManping Qian
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, 710062 China
Search for more papers by this authorCorresponding Author
Honglan Qi
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, 710062 China
E-mail: [email protected]Search for more papers by this authorQiang Gao
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, 710062 China
Search for more papers by this authorChengxiao Zhang
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, 710062 China
Search for more papers by this authorKe Wang
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, 710062 China
Search for more papers by this authorManping Qian
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, 710062 China
Search for more papers by this authorCorresponding Author
Honglan Qi
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, 710062 China
E-mail: [email protected]Search for more papers by this authorQiang Gao
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, 710062 China
Search for more papers by this authorChengxiao Zhang
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, Shaanxi, 710062 China
Search for more papers by this author†Dedicate to the Special Issue of In Situ Target Biomolecule Analysis in Confined Nanospace.
Main observation and conclusion
A single particle-based confocal laser scanning microscopy was developed for the visual detection of copper ions in confined space. A fluorescence microparticle, named AuNCs/ZIF-8, was synthesized by coating gold nanoclusters (AuNCs) onto the outer surface of zeolitic imidazolate framework-8 (ZIF-8). The morphology and solid-state fluorescence property of AuNCs/ZIF-8 were characterized by scanning electron microscopy and fluorescence technique. The AuNCs/ZIF-8 exhibits turn-off responses to copper ions via fluorescence quenching in the range of 2—15 μmol·L–1 with a detection limit of 0.9 μmol·L–1. More importantly, the distribution of AuNCs on single ZIF-8 crystal and the fluorescence quenching dynamics on single AuNCs/ZIF-8 were visualized by 3D-reconstructed fluorescence imaging. Different distribution of AuNCs on the different surfaces of single ZIF-8 crystal and similar fluorescence quenching dynamics on the different surfaces of single AuNCs/ZIF-8 were obtained. These results offer insights into the nature of the fluorescence active sites and the fluorescence quenching dynamics on a single fluorescence particle, which also opens up new opportunities for in situ target biomolecule analysis in confined space.
Supporting Information
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Appendix S1: Supporting Information |
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