Optimization of the Impeller Design for Mesenchymal Stem Cell Culture on Microcarriers in Bioreactors
Céline Loubière
Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, 54000 Nancy, France
Search for more papers by this authorAngélique Delafosse
Université de Liège, Laboratory of Chemical Engineering, Allée de la Chimie 3, B6C, 4000 Liège, Belgium
Search for more papers by this authorEmmanuel Guedon
Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, 54000 Nancy, France
Search for more papers by this authorDominique Toye
Université de Liège, Laboratory of Chemical Engineering, Allée de la Chimie 3, B6C, 4000 Liège, Belgium
Search for more papers by this authorIsabelle Chevalot
Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, 54000 Nancy, France
Search for more papers by this authorCorresponding Author
Eric Olmos
Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, 54000 Nancy, France
Correspondence: Eric Olmos ([email protected]), Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, 54000 Nancy, France.Search for more papers by this authorCéline Loubière
Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, 54000 Nancy, France
Search for more papers by this authorAngélique Delafosse
Université de Liège, Laboratory of Chemical Engineering, Allée de la Chimie 3, B6C, 4000 Liège, Belgium
Search for more papers by this authorEmmanuel Guedon
Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, 54000 Nancy, France
Search for more papers by this authorDominique Toye
Université de Liège, Laboratory of Chemical Engineering, Allée de la Chimie 3, B6C, 4000 Liège, Belgium
Search for more papers by this authorIsabelle Chevalot
Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, 54000 Nancy, France
Search for more papers by this authorCorresponding Author
Eric Olmos
Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, 54000 Nancy, France
Correspondence: Eric Olmos ([email protected]), Université de Lorraine, Laboratoire Réactions et Génie des Procédés, CNRS, LRGP, 54000 Nancy, France.Search for more papers by this authorAbstract
When agitating mesenchymal stem cells adhered on microcarriers in bioreactors, a compromise has to be found between sufficient particle suspension and limitation of hydromechanical stresses. The present study proposes a strategy to improve the design of an ‘elephant ear' impeller at the just-suspended state by varying its relative size, blade slope angle, and position in the reactor. To do that, computational fluid dynamics simulations were coupled with multi-objective optimization to minimize the hydromechanical stress encountered by the microcarriers. Two minimization criteria were considered: (P/V)@p and the energy dissipation function EDC. On the basis of 31 conditions, an optimal impeller geometry is proposed.
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