Volume 21, Issue 6 e202400619

Research Article

Metabolomic Profiling and DNA-Fingerprinting of Newly Recorded White-Flowered Populations of Salvia lanigera Poir. in Egypt

Corresponding Author

Mai M. Farid

[email protected]

orcid.org/0000-0002-4511-2781

Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, Egypt, PO Box 12622

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Mona M. Marzouk,

Mona M. Marzouk

orcid.org/0000-0003-3176-1470

Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, Egypt, PO Box 12622

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Viktoria Ivanova,

Viktoria Ivanova

orcid.org/0009-0007-9056-0333

Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

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Paraskev Nedialkov,

Paraskev Nedialkov

Pharmacognosy Department, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria

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Mona O. El Shabrawy,

Mona O. El Shabrawy

Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, Egypt, PO Box 12622

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Salwa A. Kawashty,

Salwa A. Kawashty

orcid.org/0000-0003-3914-3423

Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, Egypt, PO Box 12622

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Mahmoud M. Sakr,

Mahmoud M. Sakr

Department of Plant Biotechnology, National Research Centre, 12622 Cairo, Egypt

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Sameh R. Hussein,

Sameh R. Hussein

orcid.org/0000-0003-3657-7410

Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, Egypt, PO Box 12622

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Antoaneta Trendafilova,

Corresponding Author

Antoaneta Trendafilova

[email protected]

orcid.org/0000-0001-5273-176X

Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

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Mai M. Farid,

Corresponding Author

Mai M. Farid

[email protected]

orcid.org/0000-0002-4511-2781

Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, Egypt, PO Box 12622

Search for more papers by this author

Mona M. Marzouk,

Mona M. Marzouk

orcid.org/0000-0003-3176-1470

Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, Egypt, PO Box 12622

Search for more papers by this author

Viktoria Ivanova,

Viktoria Ivanova

orcid.org/0009-0007-9056-0333

Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

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Paraskev Nedialkov,

Paraskev Nedialkov

Pharmacognosy Department, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria

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Mona O. El Shabrawy,

Mona O. El Shabrawy

Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, Egypt, PO Box 12622

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Salwa A. Kawashty,

Salwa A. Kawashty

orcid.org/0000-0003-3914-3423

Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, Egypt, PO Box 12622

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Mahmoud M. Sakr,

Mahmoud M. Sakr

Department of Plant Biotechnology, National Research Centre, 12622 Cairo, Egypt

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Sameh R. Hussein,

Sameh R. Hussein

orcid.org/0000-0003-3657-7410

Phytochemistry and Plant Systematics Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo, Egypt, PO Box 12622

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Antoaneta Trendafilova,

Corresponding Author

Antoaneta Trendafilova

[email protected]

orcid.org/0000-0001-5273-176X

Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria

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First published: 29 April 2024

https://doi.org/10.1002/cbdv.202400619

Citations: 1

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Abstract

Salvia lanigera Poir. is a small herbaceous perennial species with violet flowers that grows in low-altitude deserts, and sandy loam. During the collection of S. lanigera, unusual populations with white flowers were found. Therefore, the two populations (violet- and white-flowered) were subjected to comparative investigations, including DNA fingerprinting, chemical composition, and biological evaluation. The two populations showed DNA variations, with 6.66 % polymorphism in ISSR and 25 % in SCoT markers. GC/MS and UHPLC/HRMS of aqueous methanol extracts, led to the tentative identification of 43 and 50 compounds in both populations. In addition, the structures of nine compounds, including four first-time reported compounds in the species, were confirmed by NMR. Furthermore, the total extracts exhibited weak radical scavenging activity against DPPH and a lower inhibitory effect towards acetylcholinesterase. In conclusion, the obtained data suggested that the white-colored flower could be an additional important character record for the Egyptian S. lanigera.

Graphical Abstract

Conflict of interests

The authors declare no conflict of interest.

Supporting Information

References

1A. F. Afonso, O. R. Pereira, S. M. Cardoso, Appl. Sci. 2021, 11, 9365.
10.3390/app11209365
Google Scholar
2A. Nasr, I. Yosuf, Z. Turki, A. Abozeid, BMC Plant Biol. 2023, 23, 1–16.
10.1186/s12870-023-04341-5
PubMed Google Scholar
3S. M. Talebi, M. Askary, N. Khalili, A. Matsyura, M. Ghorbanpour, K. Kariman, Biochem. Syst. Ecol. 2021, 96, 104269.
10.1016/j.bse.2021.104269
CAS Google Scholar
4T. A. Mohamed, I. Saleh, S. Ali, T. Hussien, N. M. Hegazi, S. Abdel-Halim, M. H. Abd El-Razek, A. El-Beih, M. M. Marzouk, P. W. Pare, J. Essent. Oil-Bear. Plants 2022, 25, 1109–1121.
10.1080/0972060X.2022.2135388
CAS Web of Science® Google Scholar
5M. B. Bahadori, B. Asghari, L. Dinparast, G. Zengin, C. Sarikurkcu, M. Abbas-Mohammadi, S. Bahadori, LWT 2017, 75, 42–50.
10.1016/j.lwt.2016.08.048
CAS Web of Science® Google Scholar
6L. Ö. Demirezer, P. Gürbüz, E. P. K. Uğur, M. Bodur, N. Özenver, A. UZ, Z. Güvenalp, Turk. J. Med. Sci. 2015, 45, 1141–1148.
10.3906/sag-1404-42
CAS PubMed Web of Science® Google Scholar
7L. Boulos, Flora of Egypt checklist, 2009.
Google Scholar
8V. Täckholm, Students’ flora of Egypt, 2nd’, Cairo University, Egypt, 1974. 888.
Google Scholar
9L. Boulos, ‘Flora of Egypt: Volume Three (Verbinaceae-Compositae)’, Al-Hadara Publishing, Cairo, Egypt, 2002. 373..
Google Scholar
10A. El-Banhawy, W. M. Kamel, E. M. G. El-din, J. Ecol. Health Environ. 2016, 4, 87–89.
10.18576/jehe/040205
Google Scholar
11N. Feinbrun-Dothan, Flora Palaestina. Part 3, 1978.
Google Scholar
12S. Duletić-Laušević, A. A. Aradski, K. Šavikin, A. Knežević, M. Milutinović, T. Stević, J. Vukojević, S. Marković, P. Marin, S. Afr. J. Bot. 2018, 117, 101–109.
10.1016/j.sajb.2018.05.013
CAS Web of Science® Google Scholar
13P. Gupta, S. Rustgi, Funct. Integr. Genomics 2004, 4, 139–162.
10.1007/s10142-004-0107-0
CAS PubMed Web of Science® Google Scholar
14V. P. Carvalho, C. F. Ruas, J. M. Ferreira, R. M. Moreira, P. M. Ruas, Genet. Mol. Biol. 2004, 27, 228–236.
10.1590/S1415-47572004000200017
CAS Web of Science® Google Scholar
15M. Farajpour, M. Ebrahimi, R. Amiri, S. Noori, S. Sanjari, R. Golzari, Afr. J. Biotechnol. 2011, 10, 11137–11141.
CAS Google Scholar
16F. Guasmi, W. Elfalleh, H. Hannachi, K. Feres, L. Touil, N. Marzougui, T. Triki, A. Ferchichi, Int. Sch. Res. Notices 2012. 2012.
Google Scholar
17A. Agarwal, V. Gupta, S. U. Haq, P. K. Jatav, S. Kothari, S. Kachhwaha, J. King Saud Univ. Sci. 2019, 31, 780–788.
10.1016/j.jksus.2018.04.022
Web of Science® Google Scholar
18U. Y. Shaheen, M. H. Hussain, H. A. Ammar, Pharmacogenomics J. 2011, 3, 36–48.
CAS Google Scholar
19A. Alimpić, N. Kotur, B. Stanković, P. D. Marin, V. Matevski, N. B. Al Sheef, S. Duletić-Lausević, JABFQ 2015, 88, 115–119.
Google Scholar
20H. Usama, E. A. Mohamed, JASMR 2006, 1, 153–159.
Google Scholar
21Z. Song, X. Li, H. Wang, J. Wang, Genetica 2010, 138, 241–249.
10.1007/s10709-009-9416-5
CAS PubMed Web of Science® Google Scholar
22R. P. Collins, Á. Helgadóttir, B. E. Frankow-Lindberg, L. Skøt, C. Jones, K. P. Skøt, Ann. Bot. 2012, 110, 1341–1350.
10.1093/aob/mcs058
PubMed Web of Science® Google Scholar
23E. Bagci, M. Vural, T. Dirmenci, L. Bruehl, K. Aitzetmüllerd, ZNC 2004, 59, 305–309.
10.1515/znc-2004-5-601
CAS PubMed Web of Science® Google Scholar
24T. Kılıç, T. Dirmenci, A. C. Gören, Rec. Nat. Prod. 2007.
Google Scholar
25A. F. Afonso, O. R. Pereira, Â. Fernandes, R. C. Calhelha, A. M. Silva, I. C. Ferreira, S. M. Cardoso, Molecules 2019, 24, 4327.
10.3390/molecules24234327
CAS PubMed Google Scholar
26N. Kharazian, F. J. Dehkordi, Z. Lorigooini, S. Afr. J. Bot. 2024, 165, 101–125.
10.1016/j.sajb.2023.12.019
CAS Web of Science® Google Scholar
27Ş. Kivrak, T. Göktürk, I. Kivrak, E. Kaya, E. Karababa, LWT 2018, 39, 423–431.
Google Scholar
28M. Mervić, M. Bival Štefan, M. Kindl, B. Blažeković, M. Marijan, S. Vladimir-Knežević, Plants 2022, 11, 625.
10.3390/plants11050625
CAS Google Scholar
29C. A. Serrano, G. K. Villena, E. F. Rodríguez, B. Calsino, M. A. Ludeña, G. V. Ccana-Ccapatinta, Sci. Rep. 2023, 13, 10714.
10.1038/s41598-023-37830-6
CAS PubMed Google Scholar
30Y. Tezuka, R. Kasimu, J. X. Li, P. Basnet, K. Tanaka, T. Namba, S. Kadota, Chem. Pharm. Bull. 1998, 46, 107–112.
10.1248/cpb.46.107
CAS Web of Science® Google Scholar
31Y. Lu, L. Y. Foo, H. Wong, Phytochemistry 1999, 52, 1149–1152.
10.1016/S0031-9422(99)00368-4
CAS Web of Science® Google Scholar
32F. Cuyckens, M. Claeys, J. Mass Spectrom. 2005, 40, 364–372.
10.1002/jms.794
CAS PubMed Web of Science® Google Scholar
33L. Feng, S. Fu, W. Du, B. Wang, L. Li, M. Zhu, C. Liu, J. Zhang, J. Chromatogr. Sci. 2015, 53, 771–777.
10.1093/chromsci/bmu124
CAS PubMed Web of Science® Google Scholar
34M. M. Marzouk, N. M. Hegazi, M. O. El Shabrawy, M. M. Farid, S. A. Kawashty, S. R. Hussein, N. A. Saleh, Metabolites 2023, 13, 909.
10.3390/metabo13080909
CAS PubMed Google Scholar
35M. M. Farid, F. M. Ibrahim, A. Y. Ragheb, R. S. Mohammed, N. M. Hegazi, M. O. E. Shabrawy, S. A. Kawashty, M. M. Marzouk, Sci. Afr. 2022, 16, e01154.
10.1016/j.sciaf.2022.e01154
CAS Google Scholar
36M. M. Marzouk, S. R. Hussein, A. Elkhateeb, M. El-shabrawy, E.-S. S Abdel-Hameed, S. A. Kawashty, J. Appl. Pharmacol. 2018, 8, 116–122.
CAS Google Scholar
37A. Ali, Y. M. Bashmil, J. J. Cottrell, H. A. Suleria, F. R. Dunshea, Antioxidants 2021, 10, 1770.
10.3390/antiox10111770
CAS PubMed Web of Science® Google Scholar
38M. Formato, S. Piccolella, C. Zidorn, A. Vastolo, S. Calabrò, M. I. Cutrignelli, S. Pacifico, Molecules 2022, 27, 2217.
10.3390/molecules27072217
CAS PubMed Google Scholar
39S. M. Bakry, A. F. A. Naser, S. I. E. Negoumy, M. E. Kassem, E. Abdel-Sattar, M. R. Meselhy, Inflammopharmacology 2023, 1–14.
Web of Science® Google Scholar
40R. Velamuri, Y. Sharma, J. Fagan, J. Schaefer, Planta Med. 2020, 7, e133–e144.
Google Scholar
41F. T. Abdl Aziz, A. S. Temraz, M. A. Hassan, AIJPMS 2024, 4, 169–188.
Google Scholar
42E. M. Kabbash, Z. T. Abdel-Shakour, S. H. El-Ahmady, M. Wink, I. M. Ayoub, Sci. Rep. 2023, 13, 612.
10.1038/s41598-022-27119-5
CAS PubMed Google Scholar
43E. Sarrou, S. Martens, P. Chatzopoulou, Ind. Crops Prod. 2016, 94, 240–250.
10.1016/j.indcrop.2016.08.022
CAS Web of Science® Google Scholar
44T. Tanaka, A. Nishimura, I. Kouno, G.-I. Nonaka, T.-J. Young, J. Nat. Prod. 1996, 59, 843–849.
10.1021/np960425s
CAS Web of Science® Google Scholar
45T. Tanaka, A. Nishimura, I. Kouno, G.-I. Nonaka, C.-R. Yang, Chem. Pharm. Bull. 1997, 45, 1596–1600.
10.1248/cpb.45.1596
CAS Web of Science® Google Scholar
46S. Simoh, ‘The effect of bacterial isochorismate synthase on the Brassica rapa metabolome’, 2008, PhD thesis, Leiden University .
Google Scholar
47M. S. Içen, İ. Gürbüz, E. Bedir, T. Günbatan, F. Demirci, S. Afr. J. Bot. 2021, 141, 177–182.
10.1016/j.sajb.2021.04.030
Web of Science® Google Scholar
48U. Y. Shaheen, Free Rad. Antiox. 2011, 1, 23–27.
10.5530/ax.2011.1.5
CAS Google Scholar
49S. M. Bakry, A. F. A. Naser, S. I. El Negoumy, M. E. Kassem, M. R. Meselhy, E. Abdel-Sattar, J. Pharm. Biomed. Anal. 2023, 235, 115620.
10.1016/j.jpba.2023.115620
CAS PubMed Google Scholar
50M. A. Asmaey, M. M. Salem, M. Emam, M. O. El Shabrawy, S. R. Hussein, A. Ibrahim, M. M. Marzouk, N. Aligiannis, M. M. Farid, S. Afr. J. Bot. 2023, 157, 209–218.
10.1016/j.sajb.2023.03.036
CAS Web of Science® Google Scholar
51R. Adams, ‘Identification of essential oil components by gas chromatography/mass spectrometry’, 2007, Allured Business Media, Carol Stream, USA.
Google Scholar
52S. Jelača, Z. Dajić-Stevanović, N. Vuković, S. Kolašinac, A. Trendafilova, P. Nedialkov, M. Stanković, N. Tanić, N. T. Tanić, A. Acović, Molecules 2022, 27, 8113.
10.3390/molecules27238113
CAS PubMed Google Scholar
53H. Wagner, S. Bladt, ‘Plant drug analysis: a thin layer chromatography atlas’, 1996, Springer Science & Business Media.
Google Scholar
54T. Ersöz, Ü. Ş. Harput, İ. Saracoğlu, İ. Çaliş, Y. Ogihara, Turk. J. Chem. 2002, 26, 581–588.
CAS Web of Science® Google Scholar
55S. B. Kim, S. H. Hwang, H.-W. Suh, S. S. Lim, Int. J. Mol. Sci. 2017, 18, 379.
10.3390/ijms18020379
PubMed Web of Science® Google Scholar
56I. B. Abdel-Farid, H. K. Kim, Y. H. Choi, R. Verpoorte, J. Agric. Food Chem. 2007, 55, 7936–7943.
10.1021/jf071294b
CAS PubMed Web of Science® Google Scholar
57E. Akoury, Am. Res. J. Chem. 2017, 2, 17–23.
Google Scholar
58Y. R. Deng, A. X. Song, H. Q. Wang, J. Chin. Chem. Soc. 2004, 51, 629–636.
10.1002/jccs.200400094
CAS Web of Science® Google Scholar
59E.-S. Hwang, N. Do Thi, Prev. Nutr. Food Sci. 2014, 19, 40.
10.3746/pnf.2014.19.1.040
PubMed Google Scholar
60J. Ferreira, S. Santos, H. Pereira, eCAM 2020, 2020.
Google Scholar

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Volume21, Issue6

June 2024

e202400619

Metabolomic Profiling and DNA-Fingerprinting of Newly Recorded White-Flowered Populations of Salvia lanigera Poir. in Egypt

Abstract

Graphical Abstract

Conflict of interests

Supporting Information

References

Citing Literature

References

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Metabolomic Profiling and DNA-Fingerprinting of Newly Recorded White-Flowered Populations of Salvia lanigera Poir. in Egypt

Abstract

Graphical Abstract

Conflict of interests

Supporting Information

References

Citing Literature

References

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