Chemical Composition, Enantiomeric Analysis, AEDA Sensorial Evaluation and Antifungal Activity of the Essential Oil from the Ecuadorian Plant Lepechinia mutica Benth (Lamiaceae)
Jorge Ramírez
Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, Loja, Ecuador
Dipartimento di Chimica and CEMEC, Università degli Studi di Pavia, Viale Taramelli 12, 27100 Pavia, Italy
Search for more papers by this authorGianluca Gilardoni
Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, Loja, Ecuador
Search for more papers by this authorMiriam Jácome
Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, Loja, Ecuador
Search for more papers by this authorJosé Montesinos
Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, Loja, Ecuador
Search for more papers by this authorMarinella Rodolfi
Department of Earth and Environmental Sciences, Lab. Mycology, University of Pavia, Via San Epifanio 14, 27100 Pavia, Italy
Search for more papers by this authorMaria Lidia Guglielminetti
Department of Earth and Environmental Sciences, Lab. Mycology, University of Pavia, Via San Epifanio 14, 27100 Pavia, Italy
Search for more papers by this authorCecila Cagliero
Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, Via Pietro Giuria 9, 10125 Torino, Italy
Search for more papers by this authorCarlo Bicchi
Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, Via Pietro Giuria 9, 10125 Torino, Italy
Search for more papers by this authorGiovanni Vidari
Dipartimento di Chimica and CEMEC, Università degli Studi di Pavia, Viale Taramelli 12, 27100 Pavia, Italy
Search for more papers by this authorJorge Ramírez
Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, Loja, Ecuador
Dipartimento di Chimica and CEMEC, Università degli Studi di Pavia, Viale Taramelli 12, 27100 Pavia, Italy
Search for more papers by this authorGianluca Gilardoni
Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, Loja, Ecuador
Search for more papers by this authorMiriam Jácome
Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, Loja, Ecuador
Search for more papers by this authorJosé Montesinos
Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja, San Cayetano Alto s/n, Loja, Ecuador
Search for more papers by this authorMarinella Rodolfi
Department of Earth and Environmental Sciences, Lab. Mycology, University of Pavia, Via San Epifanio 14, 27100 Pavia, Italy
Search for more papers by this authorMaria Lidia Guglielminetti
Department of Earth and Environmental Sciences, Lab. Mycology, University of Pavia, Via San Epifanio 14, 27100 Pavia, Italy
Search for more papers by this authorCecila Cagliero
Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, Via Pietro Giuria 9, 10125 Torino, Italy
Search for more papers by this authorCarlo Bicchi
Dipartimento di Scienza e Tecnologia del Farmaco, Università degli Studi di Torino, Via Pietro Giuria 9, 10125 Torino, Italy
Search for more papers by this authorGiovanni Vidari
Dipartimento di Chimica and CEMEC, Università degli Studi di Pavia, Viale Taramelli 12, 27100 Pavia, Italy
Search for more papers by this authorAbstract
This study describes the GC-FID, GC/MS, GC-O, and enantioselective GC analysis of the essential oil hydrodistilled from leaves of Lepechinica mutica (Lamiaceae), collected in Ecuador. GC-FID and GC/MS analyses allowed the characterization and quantification of 79 components, representing 97.3% of the total sample. Sesquiterpene hydrocarbons (38.50%) and monoterpene hydrocarbons (30.59%) were found to be the most abundant volatiles, while oxygenated sesquiterpenes (16.20%) and oxygenated monoterpenes (2.10%) were the minor components. In order to better characterize the oil aroma, the most important odorants, from the sensorial point of view, were identified by Aroma Extract Dilution Analysis (AEDA) GC-O. They were α-Pinene, β-Phellandrene, and Dauca-5,8-diene, exhibiting the characteristic woody, herbaceus, and earthy odors, respectively. Enantioselective GC analysis of L. mutica essential oil revealed the presence of twelve couples and two enantiomerically pure chiral monoterpenoids. Their enantiomeric excesses were from a few percent units to 100%. Moreover, the essential oil exhibited moderate in vitro activity against five fungal strains, being especially effective against M. canis, which is a severe zoophilic dermatophyte causal agent of pet and human infections.
Graphical Abstract
Supporting Information
Supporting information for this article is available on the WWW under https://doi.org/10.1002/cbdv.201700292.
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