Polysaccharide nanofibers and hydrogel: A comparative evaluation on 3D cell culture and tumor reduction
Preethi Gopalakrishnan Usha
Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India
Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Investigation (equal), Methodology (lead), Resources (equal), Validation (equal), Visualization (lead), Writing - original draft (lead)
Search for more papers by this authorSreekutty Jalajakumari
Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India
Contribution: Data curation (supporting), Formal analysis (supporting), Methodology (equal), Validation (supporting), Visualization (supporting)
Search for more papers by this authorUnnikrishnan Babukuttan Sheela
Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India
Contribution: Data curation (supporting), Formal analysis (supporting), Methodology (supporting), Resources (supporting), Validation (supporting), Visualization (supporting), Writing - original draft (supporting)
Search for more papers by this authorArchana Meena Gopalakrishnan
Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India
Contribution: Formal analysis (supporting), Methodology (supporting), Validation (supporting), Visualization (supporting), Writing - original draft (supporting)
Search for more papers by this authorCorresponding Author
Sreelekha Therakathinal Thankappan Nair
Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India
Correspondence
Sreelekha Therakathinal Thankappan Nair, Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Medical College P.O. Thiruvananthapuram, Kerala, India.
Email: [email protected]; [email protected]
Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Funding acquisition (lead), Investigation (equal), Methodology (supporting), Project administration (lead), Resources (equal), Supervision (lead), Validation (equal), Visualization (supporting), Writing - review & editing (lead)
Search for more papers by this authorPreethi Gopalakrishnan Usha
Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India
Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Investigation (equal), Methodology (lead), Resources (equal), Validation (equal), Visualization (lead), Writing - original draft (lead)
Search for more papers by this authorSreekutty Jalajakumari
Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India
Contribution: Data curation (supporting), Formal analysis (supporting), Methodology (equal), Validation (supporting), Visualization (supporting)
Search for more papers by this authorUnnikrishnan Babukuttan Sheela
Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India
Contribution: Data curation (supporting), Formal analysis (supporting), Methodology (supporting), Resources (supporting), Validation (supporting), Visualization (supporting), Writing - original draft (supporting)
Search for more papers by this authorArchana Meena Gopalakrishnan
Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India
Contribution: Formal analysis (supporting), Methodology (supporting), Validation (supporting), Visualization (supporting), Writing - original draft (supporting)
Search for more papers by this authorCorresponding Author
Sreelekha Therakathinal Thankappan Nair
Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Thiruvananthapuram, Kerala, India
Correspondence
Sreelekha Therakathinal Thankappan Nair, Laboratory of Biopharmaceuticals and Nanomedicine, Division of Cancer Research, Regional Cancer Centre, Medical College P.O. Thiruvananthapuram, Kerala, India.
Email: [email protected]; [email protected]
Contribution: Conceptualization (equal), Data curation (equal), Formal analysis (equal), Funding acquisition (lead), Investigation (equal), Methodology (supporting), Project administration (lead), Resources (equal), Supervision (lead), Validation (equal), Visualization (supporting), Writing - review & editing (lead)
Search for more papers by this authorAbstract
Nanofibers and hydrogels are two forms of biomaterial scaffolds that have different properties and different applications. Polysaccharides have been used for the preparation of both scaffolds due to their biocompatibility and biodegradability. In this study, our main objective was to compare the tumor cell growth and tumor reduction potential of drug-loaded scaffolds. This was achieved using different methods for cell proliferation, epithelial–mesenchymal Transition studies, and biomarker expression-related assays. Anticancer drug doxorubicin was loaded to the scaffolds using adsorption and in vitro and in vivo tumor reduction was also performed. Results showed that both scaffolds supported the growth of cancer cells but did not promote epithelial-mesenchymal transition. A significant difference in drug release was observed and this may have influenced the in vivo tumor reduction. A fast release of the drug and higher tumor-reducing potential was observed for hydrogel. The studies confirmed that different types of scaffolds have different properties. Biomedical applications related to the structure can be dissimilar in different scenarios.
Open Research
DATA AVAILABILITY STATEMENT
Research data are not shared.
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