Reversible Supramolecular Assembly at Specific DNA Sites: Nickel-Promoted Bivalent DNA Binding with Designed Peptide and Bipyridyl–Bis(benzamidine) Components†
Mateo I. Sánchez
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)
These authors contributed equally to this work.
Search for more papers by this authorJesús Mosquera
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)
These authors contributed equally to this work.
Search for more papers by this authorCorresponding Author
Prof. M. Eugenio Vázquez
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)Search for more papers by this authorCorresponding Author
Prof. José L. Mascareñas
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)Search for more papers by this authorMateo I. Sánchez
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)
These authors contributed equally to this work.
Search for more papers by this authorJesús Mosquera
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)
These authors contributed equally to this work.
Search for more papers by this authorCorresponding Author
Prof. M. Eugenio Vázquez
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)Search for more papers by this authorCorresponding Author
Prof. José L. Mascareñas
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)
Departamento de Química Orgánica and Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela (Spain)Search for more papers by this authorWe are thankful for support from Spanish grants (SAF2010-20822-C02 and CTQ2012-31341), Consolider Ingenio 2010 (CSD2007-00006), the Xunta de Galicia (GRC2013-041, INCITE09 209084PR, and PGIDIT08CSA-047209PR), the ERDF, and the European Research Council (Advanced Grant 340055). J.M. and M.I.S. thank the Spanish MCINN for their Ph.D. fellowships.
Graphical Abstract
Nickel(II) salts promote the assembly of a bis(histidine)-modified peptide that is derived from a bZIP transcription factor and a bipyridine-substituted bis(benzamidine) unit at specific DNA sites. This supramolecular system features some key properties of naturally occurring DNA-binding proteins, such as bivalence, selectivity, responsiveness to external agents, and reversibility.
Abstract
At specific DNA sites, nickel(II) salts promote the assembly of designed components, namely a bis(histidine)-modified peptide that is derived from a bZIP transcription factor and a bis(benzamidine) unit that is equipped with a bipyridine. This programmed supramolecular system with emergent properties reproduces some key characteristics of naturally occurring DNA-binding proteins, such as bivalence, selectivity, responsiveness to external agents, and reversibility.
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