Charge-Balanced Metal Fluoride Complexes for Protein Kinase A with Adenosine Diphosphate and Substrate Peptide SP20†
Dr. Yi Jin
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Search for more papers by this authorDr. Matthew J. Cliff
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Search for more papers by this authorDr. Nicola J. Baxter
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Manchester Institute of Biotechnology, Manchester, M1 7DN (UK)
Search for more papers by this authorDr. Hugh R. W. Dannatt
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Search for more papers by this authorAndrea M. Hounslow
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Search for more papers by this authorDr. Matthew W. Bowler
European Molecular Biology Laboratory and Unit of Virus Host-Cell Interactions, UJF-EMBL-CNRS, UMI 3265, 6 rue Jules Horowitz, BP 181, 38042, Grenoble (France)
Search for more papers by this authorCorresponding Author
Prof. Dr. G. Michael Blackburn
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)Search for more papers by this authorCorresponding Author
Prof. Dr. Jonathan P. Waltho
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Manchester Institute of Biotechnology, Manchester, M1 7DN (UK)
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)Search for more papers by this authorDr. Yi Jin
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Search for more papers by this authorDr. Matthew J. Cliff
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Search for more papers by this authorDr. Nicola J. Baxter
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Manchester Institute of Biotechnology, Manchester, M1 7DN (UK)
Search for more papers by this authorDr. Hugh R. W. Dannatt
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Search for more papers by this authorAndrea M. Hounslow
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Search for more papers by this authorDr. Matthew W. Bowler
European Molecular Biology Laboratory and Unit of Virus Host-Cell Interactions, UJF-EMBL-CNRS, UMI 3265, 6 rue Jules Horowitz, BP 181, 38042, Grenoble (France)
Search for more papers by this authorCorresponding Author
Prof. Dr. G. Michael Blackburn
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)Search for more papers by this authorCorresponding Author
Prof. Dr. Jonathan P. Waltho
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)
Manchester Institute of Biotechnology, Manchester, M1 7DN (UK)
Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, S10 2TN (UK)Search for more papers by this authorThe expression construct for cAPK from mouse was provided by Dr. Peter Rellos, SGC, Oxford (UK). We thank the University of Sheffield for a Studentship (to Y.J.) and BBSRC for Grant No. I002146.
Graphical Abstract
Well-balanced: 19F NMR spectroscopy defined a trifluoromagnesate complex for protein kinase A (multicolored) with adenosine diphosphate (black), the MgF3− ion (green), and the SP20 peptide substrate (purple with dots). A sphere (cyan) centered on the MgF3− ion embraces all catalytic components and much of the SP20 substrate. The content of the sphere is uncharged conforming to the charge balance hypothesis.
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