Incorporation of In Vitro Synthesized GPCR into a Tethered Artificial Lipid Membrane System†
We thank Prof. Dr. H. Ringsdorf, Prof. E. Sackmann, and Prof. L. Moroder for helpful discussions. This work was supported by a Habilitationsförderstipendium from the “Bayerisches Staatsministerium für Wissenschaft, Forschung und Kunst” and by the SFB563. GPCR=G-protein coupled receptor.
Graphical Abstract
Controlling the context: A novel strategy for the in vitro expression and immediate post-translational membrane insertion of complex membrane proteins into an artificial membrane system is described. In this way problems are circumvented that arise when complex membrane proteins are overexpressed, detergent-solubilized, and subsequently reconstituted into suitable artificial membrane systems for biophysical characterization. GPCR= G-protein coupled receptor.
