Constructing Photoactivatable Protein with Genetically Encoded Photocaged Glutamic Acid
Xiaochen Yang
State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071 China
Search for more papers by this authorLei Zhao
State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071 China
Search for more papers by this authorYing Wang
School of Life Sciences, Tianjin University, Tianjin, 300072 China
Search for more papers by this authorYanli Ji
State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071 China
Search for more papers by this authorProf. Dr. Xun-Cheng Su
State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071 China
Search for more papers by this authorProf. Dr. Jun-An Ma
Department of Chemistry, Frontier Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin, 300072 P. R. China
Search for more papers by this authorCorresponding Author
Prof. Dr. Weimin Xuan
State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071 China
School of Life Sciences, Tianjin University, Tianjin, 300072 China
Search for more papers by this authorXiaochen Yang
State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071 China
Search for more papers by this authorLei Zhao
State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071 China
Search for more papers by this authorYing Wang
School of Life Sciences, Tianjin University, Tianjin, 300072 China
Search for more papers by this authorYanli Ji
State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071 China
Search for more papers by this authorProf. Dr. Xun-Cheng Su
State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071 China
Search for more papers by this authorProf. Dr. Jun-An Ma
Department of Chemistry, Frontier Science Center for Synthetic Biology (Ministry of Education), Tianjin University, Tianjin, 300072 P. R. China
Search for more papers by this authorCorresponding Author
Prof. Dr. Weimin Xuan
State Key Laboratory and Institute of Elemento-Organic Chemistry, College of Chemistry, Nankai University, Tianjin, 300071 China
School of Life Sciences, Tianjin University, Tianjin, 300072 China
Search for more papers by this authorAbstract
Genetically replacing an essential residue with the corresponding photocaged analogues via genetic code expansion (GCE) constitutes a useful and unique strategy to directly and effectively generate photoactivatable proteins. However, the application of this strategy is severely hampered by the limited number of encoded photocaged proteinogenic amino acids. Herein, we report the genetic incorporation of photocaged glutamic acid analogues in E. coli and mammalian cells and demonstrate their use in constructing photoactivatable variants of various fluorescent proteins and SpyCatcher. We believe genetically encoded photocaged Glu would significantly promote the design and application of photoactivatable proteins in many areas.
Open Research
Data Availability Statement
The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions.
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