Selection and Characterization of an RNA-Cleaving DNAzyme Activated by Legionella pneumophila
Meghan Rothenbroker
Michael G. DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1 Canada
Search for more papers by this authorCorresponding Author
Dr. Erin M. McConnell
Michael G. DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1 Canada
Search for more papers by this authorJimmy Gu
Michael G. DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1 Canada
Search for more papers by this authorDr. Malene L. Urbanus
Department of Biochemistry, University of Toronto, Canada
Search for more papers by this authorSahar Esmaeili Samani
Department of Chemical Engineering, McMaster University, Canada
Search for more papers by this authorProf. Dr. Alex W. Ensminger
Department of Biochemistry, University of Toronto, Canada
Search for more papers by this authorProf. Dr. Carlos D. M. Filipe
Department of Chemical Engineering, McMaster University, Canada
Search for more papers by this authorCorresponding Author
Prof. Dr. Yingfu Li
Michael G. DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1 Canada
Search for more papers by this authorMeghan Rothenbroker
Michael G. DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1 Canada
Search for more papers by this authorCorresponding Author
Dr. Erin M. McConnell
Michael G. DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1 Canada
Search for more papers by this authorJimmy Gu
Michael G. DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1 Canada
Search for more papers by this authorDr. Malene L. Urbanus
Department of Biochemistry, University of Toronto, Canada
Search for more papers by this authorSahar Esmaeili Samani
Department of Chemical Engineering, McMaster University, Canada
Search for more papers by this authorProf. Dr. Alex W. Ensminger
Department of Biochemistry, University of Toronto, Canada
Search for more papers by this authorProf. Dr. Carlos D. M. Filipe
Department of Chemical Engineering, McMaster University, Canada
Search for more papers by this authorCorresponding Author
Prof. Dr. Yingfu Li
Michael G. DeGroote Institute for Infectious Disease Research, Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1 Canada
Search for more papers by this authorAbstract
Legionella pneumophila is a deadly bacterial pathogen that has caused numerous Legionnaires’ disease outbreaks, where cooling towers were the most common source of exposure. Bacterial culturing is used for L. pneumophila detection, but this method takes approximately 10 days to complete. In this work, an RNA-cleaving fluorogenic DNAzyme, named LP1, was isolated. Extensive characterization revealed that LP1 is reactive with multiple infectious isolates of L. pneumophila but inactive with 25 other common bacterial species. LP1 is likely activated by a protein target, capable of generating a detectable signal in the presence of as few as 10 colony-forming units of L. pneumophila, and able to maintain its activity in cooling tower water from diverse sources. Given that similar DNAzymes have been incorporated into many sensitive assays for bacterial detection, LP1 holds the potential for the development of biosensors for monitoring the contamination of L. pneumophila in exposure sources.
Conflict of interest
The authors declare no conflict of interest.
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