The direct delivery of specific proteins to live cells promises a tremendous impact for biological and medical applications, from therapeutics to genetic engineering. However, the process mostly involves tedious techniques and often requires extensive alteration of the protein itself. Herein we report a straightforward approach to encapsulate native proteins by using breakable organosilica matrices that disintegrate upon exposure to a chemical stimulus. The biomolecule-containing capsules were tested for the intracellular delivery of highly cytotoxic proteins into C6 glioma cells. We demonstrate that the shell is broken, the release of the active proteins occurs, and therefore our hybrid architecture is a promising strategy to deliver fragile biomacromolecules into living organisms.

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1
1aJ. Jo, S. Hong, W. Y. Choi, D. R. Lee, Sci. Rep. 2014, 4, 1–8;
Web of Science® Google Scholar
1bH. Zhou, S. Wu, J. Y. Joo, S. Zhu, D. W. Han, T. Lin, S. Trauger, G. Bien, S. Yao, Y. Zhu, G. Siuzdak, H. R. Schöler, L. Duan, S. Ding, Cell Stem Cell 2009, 4, 381–384;
10.1016/j.stem.2009.04.005
CAS PubMed Web of Science® Google Scholar
1cS. R. Schwarze, A. Ho, A. Vocero-Akbani, S. F. Dowdy, Science 1999, 285, 1569–1572.
10.1126/science.285.5433.1569
CAS PubMed Web of Science® Google Scholar
2
2aT. C. G. A. Network, Nature 2012, 490, 61–70;
10.1038/nature11412
CAS PubMed Web of Science® Google Scholar
2bA. M. Shah, D. L. Mann, Lancet 2011, 378, 704–712;
10.1016/S0140-6736(11)60894-5
CAS PubMed Web of Science® Google Scholar
2cM. Tyers, M. Mann, Nature 2003, 422, 193–197.
10.1038/nature01510
CAS PubMed Web of Science® Google Scholar
3
3aK. Fu, A. M. Klibanov, R. Langer, Nat. Biotechnol. 2000, 18, 24–25;
10.1038/71875
CAS PubMed Web of Science® Google Scholar
3bW. Jiskoot, T. W. Randolph, D. B. Volkin, C. R. Middaugh, C. Schöneich, G. Winter, W. Friess, D. J. A. Crommelin, J. F. Carpenter, J. Pharm. Sci. 2012, 101, 946–954.
10.1002/jps.23018
CAS PubMed Web of Science® Google Scholar
4
4aZ. Gu, A. Biswas, M. Zhao, Y. Tang, Chem. Soc. Rev. 2011, 40, 3638–3655;
10.1039/c0cs00227e
CAS PubMed Web of Science® Google Scholar
4bJ. Du, J. Jin, M. Yan, Y. Lu, Curr. Drug Metab. 2012, 13, 82–92.
10.2174/138920012798356862
CAS PubMed Web of Science® Google Scholar
5
5aX. Wu, Y. Song, J. Han, L. Yang, S. Han, Biomater. Sci. 2013, 1, 918;
10.1039/c3bm60128e
CAS PubMed Web of Science® Google Scholar
5bM. Wang, K. Alberti, S. Sun, C. L. Arellano, Q. Xu, Angew. Chem. Int. Ed. 2014, 53, 2893–2898;
10.1002/anie.201311245
CAS PubMed Web of Science® Google Scholar
Angew. Chem. 2014, 126, 2937–2942;
10.1002/ange.201311245
Web of Science® Google Scholar
5cH. Shen, M. Liu, Y. Chong, J. Huang, Z. Zhang, Toxicol. Res. 2013, 2, 379.
10.1039/c3tx50074h
CAS Web of Science® Google Scholar
6
6aG. Pasut, F. Veronese in Polymer Therapeutics I, Vol. 192 (Eds.: ), Springer, Berlin, 2006, pp. 95–134;
Google Scholar
6bK. Montrose, Y. Yang, X. Sun, S. Wiles, G. W. Krissansen, Sci. Rep. 2013, 3, 1661;
10.1038/srep01661
PubMed Web of Science® Google Scholar
6cS. B. Fonseca, M. P. Pereira, S. O. Kelley, Adv. Drug Delivery Rev. 2009, 61, 953–964.
10.1016/j.addr.2009.06.001
CAS PubMed Web of Science® Google Scholar
7
7aR. Tang, C. S. Kim, D. J. Solfiell, S. Rana, R. Mout, E. M. Velázquez-Delgado, A. Chompoosor, Y. Jeong, B. Yan, Z.-J. Zhu, C. Kim, J. A. Hardy, V. M. Rotello, ACS Nano 2013, 7, 6667–6673;
10.1021/nn402753y
CAS PubMed Web of Science® Google Scholar
7bP. Ghosh, X. Yang, R. Arvizo, Z.-J. Zhu, S. S. Agasti, Z. Mo, V. M. Rotello, J. Am. Chem. Soc. 2010, 132, 2642–2645;
10.1021/ja907887z
CAS PubMed Web of Science® Google Scholar
7cC. S. Kim, R. Mout, Y. Zhao, Y.-C. Yeh, R. Tang, Y. Jeong, B. Duncan, J. A. Hardy, V. M. Rotello, Bioconjugate Chem. 2015, 26, 950–954.
10.1021/acs.bioconjchem.5b00146
CAS PubMed Web of Science® Google Scholar
8
8aE. Yuba, A. Harada, Y. Sakanishi, S. Watarai, K. Kono, Biomaterials 2013, 34, 3042–3052;
10.1016/j.biomaterials.2012.12.031
CAS PubMed Web of Science® Google Scholar
8bL. J. White, G. T. S. Kirby, H. C. Cox, R. Qodratnama, O. Qutachi, F. R. A. J. Rose, K. M. Shakesheff, Mater. Sci. Eng. C 2013, 33, 2578–2583.
10.1016/j.msec.2013.02.020
CAS PubMed Web of Science® Google Scholar
9
9aM. Zhao, A. Biswas, B. Hu, K.-I. Joo, P. Wang, Z. Gu, Y. Tang, Biomaterials 2011, 32, 5223–5230;
10.1016/j.biomaterials.2011.03.060
CAS PubMed Web of Science® Google Scholar
9bT. Akagi, Y. Zhu, F. Shima, M. Akashi, Biomater. Sci. 2014, 2, 530–537;
10.1039/c3bm60279f
CAS Web of Science® Google Scholar
9cS. Santra, C. Kaittanis, J. M. Perez, Mol. Pharm. 2010, 7, 1209–1222.
10.1021/mp100043h
CAS PubMed Web of Science® Google Scholar
10M. van de Weert, W. Hennink, W. Jiskoot, Pharm. Res. 2000, 17, 1159–1167.
10.1023/A:1026498209874
CAS PubMed Web of Science® Google Scholar
11C. Chiappini, E. De Rosa, J. O. Martinez, X. Liu, J. Steele, M. M. Stevens, E. Tasciotti, Nat. Mater. 2015, 14, 532–539.
10.1038/nmat4249
CAS PubMed Web of Science® Google Scholar
12
12aC. Argyo, V. Weiss, C. Bräuchle, T. Bein, Chem. Mater. 2014, 26, 435–451;
10.1021/cm402592t
CAS Web of Science® Google Scholar
12bD. Wang, Z. Xu, Z. Chen, X. Liu, C. Hou, X. Zhang, H. Zhang, ACS Appl. Mater. Interfaces 2014, 6, 12600–12608;
10.1021/am502585x
CAS PubMed Web of Science® Google Scholar
12cY. Piao, A. Burns, J. Kim, U. Wiesner, T. Hyeon, Adv. Funct. Mater. 2008, 18, 3745–3758;
10.1002/adfm.200800731
CAS Web of Science® Google Scholar
12dM. Montalti, L. Prodi, E. Rampazzo, N. Zaccheroni, Chem. Soc. Rev. 2014, 43, 4243–4268;
10.1039/C3CS60433K
CAS PubMed Web of Science® Google Scholar
12eI. I. Slowing, B. G. Trewyn, V. S. Y. Lin, J. Am. Chem. Soc. 2007, 129, 8845–8849.
10.1021/ja0719780
CAS PubMed Web of Science® Google Scholar
13
13aK. Shah, Y. Tang, X. Breakefield, R. Weissleder, Oncogene 2003, 22, 6865–6872;
10.1038/sj.onc.1206748
CAS PubMed Web of Science® Google Scholar
13bW. Ardelt, K. Shogen, Z. Darzynkiewicz, Curr. Pharm. Biotechnol. 2008, 9, 215–225.
10.2174/138920108784567245
CAS PubMed Web of Science® Google Scholar
14
14aR. Stupp, M. E. Hegi, W. P. Mason, M. J. van den Bent, M. J. B. Taphoorn, R. C. Janzer, S. K. Ludwin, A. Allgeier, B. Fisher, K. Belanger, P. Hau, A. A. Brandes, J. Gijtenbeek, C. Marosi, C. J. Vecht, K. Mokhtari, P. Wesseling, S. Villa, E. Eisenhauer, T. Gorlia, M. Weller, D. Lacombe, J. G. Cairncross, R.-O. Mirimanoff, Lancet Oncol. 2009, 10, 459–466;
10.1016/S1470-2045(09)70025-7
CAS PubMed Web of Science® Google Scholar
14bS. Ni, X. Fan, J. Wang, H. Qi, X. Li, Ann. Biomed. Eng. 2014, 42, 214–221;
10.1007/s10439-013-0903-6
PubMed Web of Science® Google Scholar
14cZ. Lwin, D. MacFadden, A. Al-Zahrani, E. Atenafu, B. Miller, A. Sahgal, C. Menard, N. Laperriere, W. Mason, J. Neuro. Oncol. 2013, 115, 303–310.
10.1007/s11060-013-1230-3
CAS PubMed Web of Science® Google Scholar
15R. M. Kluck, E. Bossy-Wetzel, D. R. Green, D. D. Newmeyer, Science 1997, 275, 1132–1136.
10.1126/science.275.5303.1132
CAS PubMed Web of Science® Google Scholar

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Breakable Hybrid Organosilica Nanocapsules for Protein Delivery

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Breakable Hybrid Organosilica Nanocapsules for Protein Delivery

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