A Mitochondrial Surface-Specific Fluorescent Probe Activated by Bioconversion†
Corresponding Author
Dr. Yoshinori Kawazoe
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226Search for more papers by this authorDr. Hiroki Shimogawa
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226
Search for more papers by this authorDr. Ayato Sato
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226
Search for more papers by this authorCorresponding Author
Prof. Motonari Uesugi
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226
Institute for Integrated Cell-Material Sciences, Kyoto University, Uji, Kyoto 611-0011 (Japan)
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226Search for more papers by this authorCorresponding Author
Dr. Yoshinori Kawazoe
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226Search for more papers by this authorDr. Hiroki Shimogawa
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226
Search for more papers by this authorDr. Ayato Sato
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226
Search for more papers by this authorCorresponding Author
Prof. Motonari Uesugi
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226
Institute for Integrated Cell-Material Sciences, Kyoto University, Uji, Kyoto 611-0011 (Japan)
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011 (Japan), Fax: (+81)774-38-3226Search for more papers by this authorThis work was supported by the JST, the Uehara Memorial Foundation, and the Global COE program.
Graphical Abstract
Beim zellbasierten Screening von 12 000 niedermolekularen Verbindungen mit aromatischen Gruppen wurden 31 Moleküle als potenzielle Fluoreszenzsonden identifiziert. Einer der Kandidaten ist die erste Fluoreszenzsonde, die mitochondriale Oberflächen spezifisch anfärbt. Spektroskopische Analysen zeigen, dass das Molekül durch eine Bioumwandlung in der Zelle fluoreszierend wird.
Supporting Information
Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors.
| Filename | Description |
|---|---|
| ange_201100935_sm_miscellaneous_information.pdf3.9 MB | miscellaneous_information |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
References
- 1S. Adams, A. Harootunian, Y. Buechler, S. Taylor, R. Tsien, Nature 1991, 349, 694–697.
- 2
- 2aD. Perez-Sala, D. Collado-Escobar, F. Mollinedo, J. Biol. Chem. 1995, 270, 6235–6242;
- 2bE. Adie, S. Kalinka, L. Smith, M. Francis, A. Marenghi, M. Cooper, M. Briggs, N. Michael, G. Milligan, S. Game, Biotechniques 2002, 33, 1152–1154.
- 3
- 3aH. Zhang, J. Joseph, C. Felix, B. Kalyanaraman, J. Biol. Chem. 2000, 275, 14038–14045;
- 3bT. Iuchi, M. Akaike, T. Mitsui, Y. Ohshima, Y. Shintani, H. Azuma, T. Matsumoto, Circ. Res. 2003, 92, 81–87.
- 4
- 4aY. Tsien, T. Rink, M. Poenie, Cell Calcium 1985, 6, 145–157;
- 4bP. Negulescu, A. Harootunian, R. Tsien, T. Machen, Cell Regul. 1990, 1, 259–268;
- 4cS. Burdette, G. Walkup, B. Spingler, R. Tsien, S. Lippard, J. Am. Chem. Soc. 2001, 123, 7831–7841;
- 4dK. Kikuchi, K. Komatsu, T. Nagano, Curr. Opin. Chem. Biol. 2004, 8, 182–191.
- 5H. Kojima, K. Sakurai, K. Kikuchi, S. Kawahara, Y. Kirino, H. Nagoshi, Y. Hirata, T. Akaike, H. Maeda, T. Nagano, Biol. Pharm. Bull. 1997, 20, 1229–1232.
- 6S. Wang, Y. T. Chang, Chem. Commun. 2008, 1173–1175.
- 7
- 7aS. Feng, Y. K. Kim, Y. T. Chang, Chem. Commun. 2010, 46, 436–438;
- 7bQ. Li, Y. Kim, J. Namm, A. Kulkami, G. R. Rosania, Y. H. Ahn, Y. T. Chang, Chem. Biol. 2006, 13, 615–623.
- 8C. N. Im, N. Y. Kang, H. H. Ha, X. Bi, J. J. Lee, S. J. Park, S. Y. Lee, M. Vendrell, Y. K. Kim, J. S. Lee, J. Li, Y. H. Ahn, B. Feng, H. H. Ng, S. W. Yun, Y. T. Chang, Angew. Chem. 2010, 122, 7659–7662; Angew. Chem. Int. Ed. 2010, 49, 7497–7500.
- 9
- 9aR. Tsien, Nat. Rev. Mol. Cell. Biol. 2003, Suppl, SS 16–21;
- 9bM. Vendrell, J. S. Lee, Y. T. Chang, Curr. Opin. Chem. Biol. 2010, 14, 383–389.
- 10M. F. C. Abad, G. D. Benedetto, P. J. Magalhaes, L. Filippin, T. Pozzan, J. Biol. Chem. 2004, 279, 11521–11529.
- 11
- 11aG. Grynkiewicz, M. Poenie, R. Y. Tsien, J. Biol. Chem. 1985, 260, 3440–3450;
- 11bS. Izumi, Y. Urano, K. Hanaoka, T. Terai, T. Nagano, J. Am. Chem. Soc. 2009, 131, 10189–10200.
- 12
- 12aD. R. Green, J. C. Reed, Science 1998, 281, 1309–1312;
- 12bN. A. Thornberry, Y. Lazebnik, Science 1998, 281, 1312–1316;
- 12cA. Gross, J. M. McDonnell, S. J. Korsmeyer, Genes Dev. 1999, 13, 1899–1991.
- 13
- 13aR. Rizzuto, P. Pinton, W. Carrington, F. S. Fay, K. E. Forgarty, L. M. Lifshitz, R. A. Tuft, T. Pozzan, Science 1998, 280, 1763–1766;
- 13bS. J. Stone, J. E. Vance, J. Biol. Chem. 2000, 275, 34534–34540;
- 13cC. Giorgi, D. D. Stefani, A. Bononi, R. Rizzuto, P. Pinton, Int. J. Biochem. Cell Biol. 2009, 41, 1817–1827.
- 14E. Kim, M. Koh, J. Ryu, S. B. Park, J. Am. Chem. Soc. 2008, 130, 12206–12207.
Citing Literature
This is the
German version
of Angewandte Chemie.
Note for articles published since 1962:
Do not cite this version alone.
Take me to the International Edition version with citable page numbers, DOI, and citation export.
We apologize for the inconvenience.
