Ultrasensitive Detection of microRNAs by Exponential Isothermal Amplification†
Hongxia Jia
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province (P. R. China), Fax: (+86) 312-507-9403
Search for more papers by this authorZhengping Li Prof. Dr.
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province (P. R. China), Fax: (+86) 312-507-9403
Search for more papers by this authorChenghui Liu
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province (P. R. China), Fax: (+86) 312-507-9403
Search for more papers by this authorYongqiang Cheng
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province (P. R. China), Fax: (+86) 312-507-9403
Search for more papers by this authorHongxia Jia
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province (P. R. China), Fax: (+86) 312-507-9403
Search for more papers by this authorZhengping Li Prof. Dr.
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province (P. R. China), Fax: (+86) 312-507-9403
Search for more papers by this authorChenghui Liu
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province (P. R. China), Fax: (+86) 312-507-9403
Search for more papers by this authorYongqiang Cheng
Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry and Environmental Science, Hebei University, Baoding 071002, Hebei Province (P. R. China), Fax: (+86) 312-507-9403
Search for more papers by this authorThis project was supported by the National Natural Science Foundation of China (20925519, 20875021) and the National Science Foundation of Hebei Province (B2009001525).
Graphical Abstract
Klitzekleine miRNA-Mengen (bis hinab zu 0.1 zmol) lassen sich exakt und in Echtzeit quantitativ bestimmen. Die hierfür eingesetzte Methode beruht auf einer exponentiellen Vervielfältigungsreaktion (siehe Bild) mit einem dynamischen Bereich über 10 Größenordnungen. Die Reaktion dauert nur 30 min, benötigt weder Temperaturwechsel noch modifizierte DNA-Sonden und erkennt miRNA-Sequenzen, die sich in nur einer Base unterscheiden.
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