Chapter 19
Liquid Chromatography Mass Spectrometry Bioanalysis of Protein Therapeutics and Biomarkers in Biological Matrices
Fumin Li,
Qin C. Ji,
Fumin Li
Bioanalytical Department, Covance Laboratories, Madison, Wisconsin, USA
Search for more papers by this authorQin C. Ji
Bioanalytical Sciences, Analytical Research & Development, Bristol-Myers Squibb, Princeton, New Jersey, USA
Search for more papers by this authorFumin Li,
Qin C. Ji,
Fumin Li
Bioanalytical Department, Covance Laboratories, Madison, Wisconsin, USA
Search for more papers by this authorQin C. Ji
Bioanalytical Sciences, Analytical Research & Development, Bristol-Myers Squibb, Princeton, New Jersey, USA
Search for more papers by this authorBook Editor(s):Mike S. Lee, Mingshe Zhu,
Mingshe Zhu
Department of Biotransformation, Bristol-Myers Squibb Research and Development, Princeton, New Jersey, USA
Search for more papers by this authorSeries Editor(s): Mike S. Lee,
Summary
This chapter contains sections titled:
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General Introduction
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Protein Quantitation by LC–MS/MS
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Protein Quantitation Using Intact Proteins by LC–MS/MS
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Protein Quantitation Using Representative Peptides by LC–MS/MS
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Consideration of Internal Standard for Protein Quantitation
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Matrix Effect, Matrix Suppression/Enhancement, and Recovery
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Sensitivity Enhancement via Immunocapture/Purification
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Sensitivity Enhancement via Depletion of Abundant Proteins
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Practical Aspects of LC–MS Assay for Proteins in Drug Development
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Conclusions
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Acknowledgments
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References
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