Quorum sensing inhibitory effect of hexanal on Autoinducer-2 (AI-2) and corresponding impacts on biofilm formation and enzyme activity in Erwinia carotovora and Pseudomonas fluorescens isolated from vegetables
Ying Zhang
School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, China
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Data curation, Formal analysis, Writing - original draft
Search for more papers by this authorHang Yu
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Joint International Research Laboratory of Food Safety, Jiangnan University, Wuxi, China
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, Jiangnan University, Wuxi, China
Contribution: Investigation, Software, Writing - review & editing
Search for more papers by this authorYunfei Xie
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Joint International Research Laboratory of Food Safety, Jiangnan University, Wuxi, China
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, Jiangnan University, Wuxi, China
Contribution: Data curation, Project administration
Search for more papers by this authorYahui Guo
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Joint International Research Laboratory of Food Safety, Jiangnan University, Wuxi, China
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, Jiangnan University, Wuxi, China
Contribution: Investigation, Validation
Search for more papers by this authorYuliang Cheng
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Joint International Research Laboratory of Food Safety, Jiangnan University, Wuxi, China
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, Jiangnan University, Wuxi, China
Contribution: Conceptualization
Search for more papers by this authorCorresponding Author
Weirong Yao
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Joint International Research Laboratory of Food Safety, Jiangnan University, Wuxi, China
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, Jiangnan University, Wuxi, China
Correspondence
Weirong Yao, State Key Laboratory of Food Science and Technology, Jiangnan University, No. 1800 Lihu Avenue, Wuxi, Jiangsu Province, 214122, China.
Email: [email protected]
Contribution: Funding acquisition, Supervision
Search for more papers by this authorYing Zhang
School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai, China
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Contribution: Data curation, Formal analysis, Writing - original draft
Search for more papers by this authorHang Yu
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Joint International Research Laboratory of Food Safety, Jiangnan University, Wuxi, China
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, Jiangnan University, Wuxi, China
Contribution: Investigation, Software, Writing - review & editing
Search for more papers by this authorYunfei Xie
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Joint International Research Laboratory of Food Safety, Jiangnan University, Wuxi, China
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, Jiangnan University, Wuxi, China
Contribution: Data curation, Project administration
Search for more papers by this authorYahui Guo
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Joint International Research Laboratory of Food Safety, Jiangnan University, Wuxi, China
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, Jiangnan University, Wuxi, China
Contribution: Investigation, Validation
Search for more papers by this authorYuliang Cheng
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Joint International Research Laboratory of Food Safety, Jiangnan University, Wuxi, China
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, Jiangnan University, Wuxi, China
Contribution: Conceptualization
Search for more papers by this authorCorresponding Author
Weirong Yao
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China
School of Food Science and Technology, Jiangnan University, Wuxi, China
Joint International Research Laboratory of Food Safety, Jiangnan University, Wuxi, China
National Center for Technology Innovation on Fast Biological Detection of Grain Quality and Safety, Jiangnan University, Wuxi, China
Correspondence
Weirong Yao, State Key Laboratory of Food Science and Technology, Jiangnan University, No. 1800 Lihu Avenue, Wuxi, Jiangsu Province, 214122, China.
Email: [email protected]
Contribution: Funding acquisition, Supervision
Search for more papers by this authorAbstract
Hexanal is an essential oil component which has quorum sensing (QS) inhibitor effect on vegetable spoilage bacteria such as Erwinia carotovora (E. carotovora) and Pseudomonas fluorescens (P. fluorescens). However, the QS inhibitory (QSI) effect of hexanal on Autoinducer-2 (AI-2), biofilm formation, and enzyme activity is not well understood. Our results showed that the contents of AI-2 produced by E. carotovora were higher than P. fluorescens, and the 4,5-Dihydroxy-2,3-pentanedione (DPD) concentrations were significantly (p < .05) inhibited by hexanal. Adding DPD exogenously could form more biofilm when inhibited by 1/2 of minimum inhibitory concentration (MIC) of hexanal, which showed that QSI effect of hexanal on biofilm was due to its inhibition on AI-2. There was no significant increase in enzyme activity, which suggested that enzyme inhibition by hexanal may not due to AI-2. Understanding the relationship between QSI effect of hexanal on AI-2, biofilm formation, and enzyme activity can improve to suppress decomposition of vegetables after harvest.
Practical applications
Hexanal is an EO component which has shown good QSI effect on biofilm formation and exoenzyme activities of P. fluorescens and E. carotovora. However, the reason for the QSI effect of hexanal was still unclear. In this study, hexanal as a QS inhibitor on these two vegetable spoilage bacteria was studied from the perspective of AI-2 signal molecule. Understanding the QS inhibition of hexanal on AI-2 signal molecule of spoilage bacteria and its relationship with biofilm formation and enzyme activity was necessary for the application of more EO components as QS inhibitor to suppress corruption of vegetables after harvest.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
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