Comparative analysis of dermal collagen and lipids in cereblon ablated mice using a multimodal nonlinear optical system
Chang Woo Park and Seungje Jeon contributed equally to this work.
Funding information: Gwangju Institute of Science and Technology, Grant/Award Numbers: GIST Research Institute (GRI) Grant, Quantum Beam Facility Program; Inje University, Grant/Award Number: Inje University Research Grant
Abstract
By utilizing a multimodal nonlinear optical system that combines coherent anti-Stokes Raman scattering and second harmonic generation to investigate biological characteristics of dermal tissues ex vivo, we demonstrate the potential feasibility of using this optical approach as a powerful new investigative tool for future biomedical research. For this study, our optical system was utilized for the first time to analyze lipid and collagen profiles in cereblon knockout (KO) mouse skin, and we were able to discover significant alterations in the number of carbon–carbon double bonds (wild-type vs. cereblon KO; NCC: 0.75 vs. 0.85) of skin fatty acids in triacylglycerides as well as changes in dermal collagen fibers (25% reduction in cereblon KO). By adopting our optical system to biological studies, we provide researchers with another diagnostic approach to validate their experimental results, which will significantly advance the state of biomedical research.
CONFLICTS OF INTEREST
The authors declare no conflict of interests.
Open Research
DATA AVAILABILITY STATEMENT
The data that support the findings of this study are available from the corresponding author upon reasonable request.
