Volume 13, Issue 10 e202000090

FULL ARTICLE

Visibility of microvessels in Optical Coherence Tomography angiography depends on angular orientation

Jun Zhu,

Jun Zhu

orcid.org/0000-0002-7905-7856

Biomedical Engineering Department, University of California Davis, Davis, California, USA

Jun Zhu and Marcel T. Bernucci contributed equally to this work.

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Marcel T. Bernucci,

Marcel T. Bernucci

Biomedical Engineering Department, University of California Davis, Davis, California, USA

Jun Zhu and Marcel T. Bernucci contributed equally to this work.

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Conrad W. Merkle,

Conrad W. Merkle

Biomedical Engineering Department, University of California Davis, Davis, California, USA

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Vivek J. Srinivasan,

Corresponding Author

Vivek J. Srinivasan

[email protected]

Biomedical Engineering Department, University of California Davis, Davis, California, USA

Department of Ophthalmology and Vision Science, University of California Davis School of Medicine, Sacramento, California, USA

Correspondence

Vivek J. Srinivasan, Biomedical Engineering Department, University of California Davis, 451 E. Health Sciences Dr., Davis, CA 95616, USA.

Email: [email protected]

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Jun Zhu,

Jun Zhu

orcid.org/0000-0002-7905-7856

Biomedical Engineering Department, University of California Davis, Davis, California, USA

Jun Zhu and Marcel T. Bernucci contributed equally to this work.

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Marcel T. Bernucci,

Marcel T. Bernucci

Biomedical Engineering Department, University of California Davis, Davis, California, USA

Jun Zhu and Marcel T. Bernucci contributed equally to this work.

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Conrad W. Merkle,

Conrad W. Merkle

Biomedical Engineering Department, University of California Davis, Davis, California, USA

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Vivek J. Srinivasan,

Corresponding Author

Vivek J. Srinivasan

[email protected]

Biomedical Engineering Department, University of California Davis, Davis, California, USA

Department of Ophthalmology and Vision Science, University of California Davis School of Medicine, Sacramento, California, USA

Correspondence

Vivek J. Srinivasan, Biomedical Engineering Department, University of California Davis, 451 E. Health Sciences Dr., Davis, CA 95616, USA.

Email: [email protected]

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First published: 29 May 2020

https://doi.org/10.1002/jbio.202000090

Citations: 9

Funding information: National Institutes of Health, Grant/Award Numbers: R01EY028287, R01EY030361, R01EY031469, R01NS094681, R01EB029747, R21NS105043

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Abstract

Optical Coherence Tomography angiography (OCTA) is a widespread tool for depth-resolved imaging of chorioretinal vasculature with single microvessel resolution. To improve the clinical interpretation of OCTA, the conditions affecting visualization of microvessels must be defined. Here we inject a scattering plasma tracer (Intralipid) during OCTA imaging of the anesthetized rat eye. In the retina, we find that interlaminar (vertical) vessels that connect laminae have one-fourth to one-third the OCTA red blood cell to tracer (RBC-to-tracer) signal ratio of intralaminar (horizontal) vessels. This finding suggests that the OCTA signal from microvessels depends on angular orientation, making vertically-oriented vessels more difficult to visualize using intrinsic contrast alone. Clinicians should be aware of this potential artifact when interpreting OCTA.

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Volume13, Issue10

October 2020

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Visibility of microvessels in Optical Coherence Tomography angiography depends on angular orientation

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Visibility of microvessels in Optical Coherence Tomography angiography depends on angular orientation

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