Pathological crystal imaging with single-shot computational polarized light microscopy
Bijie Bai
Electrical and Computer Engineering Department, University of California, Los Angeles, Los Angeles, California
Bioengineering Department, University of California, Los Angeles, Los Angeles, California
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California
Search for more papers by this authorHongda Wang
Electrical and Computer Engineering Department, University of California, Los Angeles, Los Angeles, California
Bioengineering Department, University of California, Los Angeles, Los Angeles, California
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California
Search for more papers by this authorTairan Liu
Electrical and Computer Engineering Department, University of California, Los Angeles, Los Angeles, California
Bioengineering Department, University of California, Los Angeles, Los Angeles, California
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California
Search for more papers by this authorYair Rivenson
Electrical and Computer Engineering Department, University of California, Los Angeles, Los Angeles, California
Bioengineering Department, University of California, Los Angeles, Los Angeles, California
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California
Search for more papers by this authorJohn FitzGerald
Division of Rheumatology, Department of Internal Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California
Search for more papers by this authorCorresponding Author
Aydogan Ozcan
Electrical and Computer Engineering Department, University of California, Los Angeles, Los Angeles, California
Bioengineering Department, University of California, Los Angeles, Los Angeles, California
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California
Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California
Correspondence
Aydogan Ozcan, University of California, Los Angeles, CA 90095.
Email: [email protected]
Search for more papers by this authorBijie Bai
Electrical and Computer Engineering Department, University of California, Los Angeles, Los Angeles, California
Bioengineering Department, University of California, Los Angeles, Los Angeles, California
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California
Search for more papers by this authorHongda Wang
Electrical and Computer Engineering Department, University of California, Los Angeles, Los Angeles, California
Bioengineering Department, University of California, Los Angeles, Los Angeles, California
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California
Search for more papers by this authorTairan Liu
Electrical and Computer Engineering Department, University of California, Los Angeles, Los Angeles, California
Bioengineering Department, University of California, Los Angeles, Los Angeles, California
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California
Search for more papers by this authorYair Rivenson
Electrical and Computer Engineering Department, University of California, Los Angeles, Los Angeles, California
Bioengineering Department, University of California, Los Angeles, Los Angeles, California
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California
Search for more papers by this authorJohn FitzGerald
Division of Rheumatology, Department of Internal Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California
Search for more papers by this authorCorresponding Author
Aydogan Ozcan
Electrical and Computer Engineering Department, University of California, Los Angeles, Los Angeles, California
Bioengineering Department, University of California, Los Angeles, Los Angeles, California
California NanoSystems Institute, University of California, Los Angeles, Los Angeles, California
Department of Surgery, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, California
Correspondence
Aydogan Ozcan, University of California, Los Angeles, CA 90095.
Email: [email protected]
Search for more papers by this authorFunding information: National Institutes of Health, Grant/Award Number: R21AR072946
Abstract
Pathological crystal identification is routinely practiced in rheumatology for diagnosing arthritis disease such as gout, and relies on polarized light microscopy as the gold standard method used by medical professionals. Here, we present a single-shot computational polarized light microscopy method that reconstructs the transmittance, retardance and slow-axis orientation of a birefringent sample using a single image captured with a pixelated-polarizer camera. This method is fast, simple-to-operate and compatible with all the existing standard microscopes without extensive or costly modifications. We demonstrated the success of our method by imaging three different types of crystals found in synovial fluid and reconstructed the birefringence information of these samples using a single image, without being affected by the orientation of individual crystals within the sample field-of-view. We believe this technique will provide improved sensitivity, specificity and speed, all at low cost, for clinical diagnosis of crystals found in synovial fluid and other bodily fluids.
CONFLICT OF INTEREST
A.O., B.B., and H.W. have a pending patent application on the presented technique. The remaining authors declare that they have no conflict of interest.
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