Morphological and functional characteristics of aging kidneys based on two-photon microscopy in vivo
Shulian Wu
Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou, China
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Search for more papers by this authorHengchang Guo
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Search for more papers by this authorHannah Horng
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Search for more papers by this authorYi Liu
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Search for more papers by this authorHui Li
Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou, China
Search for more papers by this authorParnaz Daneshpajouhnejad
Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorAvi Rosenberg
Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorChristopher Albanese
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC
Search for more papers by this authorSuman Ranjit
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC
Search for more papers by this authorPeter M. Andrews
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC
Search for more papers by this authorMoshe Levi
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC
Search for more papers by this authorCorresponding Author
Qinggong Tang
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma
Correspondence
Yu Chen, Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742.
Email: [email protected]
Qinggong Tang, Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, OK 73019.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Yu Chen
Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou, China
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Correspondence
Yu Chen, Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742.
Email: [email protected]
Qinggong Tang, Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, OK 73019.
Email: [email protected]
Search for more papers by this authorShulian Wu
Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou, China
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Search for more papers by this authorHengchang Guo
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Search for more papers by this authorHannah Horng
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Search for more papers by this authorYi Liu
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Search for more papers by this authorHui Li
Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou, China
Search for more papers by this authorParnaz Daneshpajouhnejad
Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorAvi Rosenberg
Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland
Search for more papers by this authorChristopher Albanese
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC
Search for more papers by this authorSuman Ranjit
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC
Search for more papers by this authorPeter M. Andrews
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC
Search for more papers by this authorMoshe Levi
Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, Washington, DC
Search for more papers by this authorCorresponding Author
Qinggong Tang
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, Oklahoma
Correspondence
Yu Chen, Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742.
Email: [email protected]
Qinggong Tang, Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, OK 73019.
Email: [email protected]
Search for more papers by this authorCorresponding Author
Yu Chen
Fujian Provincial Key Laboratory of Photonic Technology, Key Laboratory of Optoelectronic Science and Technology for Medicine, College of Photonic and Electronic Engineering, Fujian Normal University, Ministry of Education, Fuzhou, China
Fischell Department of Bioengineering, University of Maryland, College Park, Maryland
Correspondence
Yu Chen, Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742.
Email: [email protected]
Qinggong Tang, Stephenson School of Biomedical Engineering, University of Oklahoma, Norman, OK 73019.
Email: [email protected]
Search for more papers by this authorFunding information: Foundation for the National Institutes of Health, Grant/Award Number: R21 AG042700; Special Funds of the Central Government Guiding Local Science and Technology Development, Grant/Award Number: 2017L3009
Abstract
Age-related kidney disease, which is chronic and naturally occurring, is a general term for a set of heterogeneous disorders affecting kidney structures and characterized by a decline in renal function. Age-related renal insufficiency has important implications with regard to body homeostasis, drug toxicity and renal transplantation. In our study, two-photon microscopy was used to image kidney morphological and functional characteristics in an age-related rat model in vivo. The changes in morphology are analyzed based on autofluorescence and Hoechst 33342 labeling in rats with different ages. Structural parameters including renal tubular diameter, cell nuclei density, size and shape are studied and compared with Hematoxylin and Eosin histological analysis. Functional characteristics, such as blood flow, and glomerular filtration rate are studied with high-molecular weight (MW) 500-kDa dextran-fluorescein and low-MW 10-kDa dextran-rhodamine. Results indicate that morphology changes significantly and functional characteristics deteriorate with age. These parameters are potential indicators for evaluating age-related renal morphology and function changes. Combined analyses of these parameters could provide a quantitative, novel method for monitoring kidney diseases and/or therapeutic effects of kidney drugs.
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