ORIGINAL ARTICLE
Ultrasound assisted extraction of phenolic compounds from Capparis Ovata var canescens fruit using deep eutectic solvents
Sercan Ozbek Yazici,
İsmail Ozmen,
Corresponding Author
Sercan Ozbek Yazici
Faculty of Health Sciences, Department of Nutrition and Dietetics, Burdur Mehmet Akif Ersoy University, Burdur, Turkey
Correspondence
Sercan Ozbek Yazici, Faculty of Health Sciences, Department of Nutrition and Dietetics, Burdur Mehmet Akif Ersoy University, Istiklal Yerleskesi, Burdur, 15100, Turkey.
Email: [email protected]
Contribution: Conceptualization, Formal analysis, Investigation, Methodology, Writing - original draft
Search for more papers by this authorİsmail Ozmen
Art and Science Faculty, Department of Chemistry, Suleyman Demirel University, Isparta, Turkey
Contribution: Formal analysis, Methodology, Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorSercan Ozbek Yazici,
İsmail Ozmen,
Corresponding Author
Sercan Ozbek Yazici
Faculty of Health Sciences, Department of Nutrition and Dietetics, Burdur Mehmet Akif Ersoy University, Burdur, Turkey
Correspondence
Sercan Ozbek Yazici, Faculty of Health Sciences, Department of Nutrition and Dietetics, Burdur Mehmet Akif Ersoy University, Istiklal Yerleskesi, Burdur, 15100, Turkey.
Email: [email protected]
Contribution: Conceptualization, Formal analysis, Investigation, Methodology, Writing - original draft
Search for more papers by this authorİsmail Ozmen
Art and Science Faculty, Department of Chemistry, Suleyman Demirel University, Isparta, Turkey
Contribution: Formal analysis, Methodology, Supervision, Writing - original draft, Writing - review & editing
Search for more papers by this authorAbstract
The aim of this study is to develop a green procedure that provides high-efficiency extraction of phenolic compounds from Capparis Ovata fruit with Deep Eutectic Solvent (DES) using ultrasound-assisted extraction method. Ten DES combinations were prepared by mixing various proportions of choline chloride (ChCl), Na-acetate and Na-citrate with sugar alcohols, carboxylic acid. These DESs were tested as an effective solvent for recovering phenolic compounds. DES composed of lactic acid combining with ChCl showed the highest efficiency of extraction for phenolic compounds. Optimal extraction conditions were found as follows; 20% water in DES, liquid/solid ratio 20:1 (mL/g), temperature 50℃, and extraction time 30 min. The maximum total phenolic content, total flavonoid content, DPPH inhibition percentage, and ferric reducing power capacity values under optimal conditions were 30.55 ± 0.57 mg GAE/g DW, 13.04 ± 0.4 mg QE/g DW, 98.49 ± 2.51% and 1.79 ± 0.04 (absorbance units), respectively. The concentrations of all phenolic compounds determined by chromatographic method have increased under optimal extraction conditions.
Practical applications
The application of DES in the extraction of the phenolic compound is an innovative approach for environmentally, friendly, low-costs, and improving extraction efficiency. Caper fruits are known to be rich sources of bioactive compounds. Caper is consumed as food and traditional folk medicines for their health benefits. At previous studies, a green extraction procedure to obtain phenolic compounds from Capparis Ovata var canescens fruit has not been established by using DES. Therefore, this study aimed to improve a green procedure to obtain phenolic compounds with strong antioxidant properties, which could be employed in the food industry. In addition, determination of optimal extraction conditions has been performed to guide large-scale production.
CONFLICT OF INTEREST
The authors have declared no conflicts of interest for this article.
Open Research
DATA AVAILABILITY STATEMENT
Research data are not shared.
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