• Issue

    Protein Science: Volume 30, Issue 6

    1099-1269
    June 2021
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Cover Image

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Cover Image

  • First Published: 21 May 2021
Cover Image

Yellow bacteria isolated from the lips of the artist Maria Penil Cobo were applied to make an image of antibodies on a petri dish and allowed to grow. Copper wires were added after growth to represent protein alpha-helices and betastrands. This image was inspired by the artist working in the laboratory of Dr. Berkmen while the data represented in this manuscript were being acquired. For more information, visit www.bacterialart.com. See article by Lénon et al. pp. 1235–1246.

Issue Information

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Issue information

  • Pages: 1099-1101
  • First Published: 21 May 2021

In This Issue

Free Access

In This Issue

  • Page: 1102
  • First Published: 21 May 2021

Reviews

Open Access

Prokaryote toxin–antitoxin modules: Complex regulation of an unclear function

Pieter De Bruyn,  Yana Girardin,  Remy Loris, 
  • Pages: 1103-1113
  • First Published: 31 March 2021
Free Access

Characterization of the SARS-CoV-2 E Protein: Sequence, Structure, Viroporin, and Inhibitors

Yipeng Cao,  Rui Yang,  Imshik Lee,  Wenwen Zhang,  Jiana Sun,  Wei Wang,  Xiangfei Meng, 
  • Pages: 1114-1130
  • First Published: 04 April 2021

Full-Length Papers

Free Access

Multiple sites on SARS-CoV-2 spike protein are susceptible to proteolysis by cathepsins B, K, L, S, and V

Keval Bollavaram,  Tiffanie H. Leeman,  Maggie W. Lee,  Akhil Kulkarni,  Sophia G. Upshaw,  Jiabei Yang,  Hannah Song,  Manu O. Platt, 
  • Pages: 1131-1143
  • First Published: 31 March 2021

PDB Code(s): 6VYB, 4Z2A, 5F02, 4P6E, 5TUN, 2IPP and 3H6S;

Free Access

An ultra-high affinity protein–protein interface displaying sequence-robustness

Marie Sofie Møller,  Sita Vaag Olesen,  Ingemar André, 
  • Pages: 1144-1156
  • First Published: 10 April 2021
Free Access

Dynamically probing ATP-dependent RNA helicase A-assisted RNA structure conversion using single molecule fluorescence resonance energy transfer

Chengcheng Wu,  Yanke Shan,  Shouyu Wang,  Fei Liu, 
  • Pages: 1157-1168
  • First Published: 10 April 2021
Free Access

High-throughput identification of conditional MHCI ligands and scaled-up production of conditional MHCI complexes

Martine Darwish,  Sara Wichner,  Jenny Li,  Jiun Chiun Chang,  Christine Tam,  Yvonne Franke,  Hong Li,  Pamela Chan,  Craig Blanchette, 
  • Pages: 1169-1183
  • First Published: 11 April 2021
Free Access

Human DND1-RRM2 forms a non-canonical domain swapped dimer

Pooja Kumari,  Neel Sarovar Bhavesh, 
  • Pages: 1184-1195
  • First Published: 16 April 2021

PDB Code(s): 6LE1;

Open Access

Crystal structure and molecular dynamics of human POLDIP2, a multifaceted adaptor protein in metabolism and genome stability

Anastasija A. Kulik,  Klaudia K. Maruszczak,  Dana C. Thomas,  Naomi L. A. Nabi-Aldridge,  Martin Carr,  Richard J. Bingham,  Christopher D. O. Cooper, 
  • Pages: 1196-1209
  • First Published: 21 April 2021

PDB Code(s): 6Z9C;

Free Access

Structural and mechanistic insight into stem-loop RNA processing by yeast Pichia stipitis Dicer

JingRu Chan,  Fu Qinqin,  Li Jianwei,  Chen Ying,  Satoru Machida,  Chen Wei,  Yuren Adam Yuan,  Chacko Jobichen, 
  • Pages: 1210-1220
  • First Published: 21 April 2021

PDB Code(s): 7DEY;

Free Access

Structural dynamics of the complex of calmodulin with a minimal functional construct of eukaryotic elongation factor 2 kinase and the role of Thr348 autophosphorylation

Andrea Piserchio,  Kimberly Long,  Kwangwoon Lee,  Eric A. Kumar,  Rinat Abzalimov,  Kevin N. Dalby,  Ranajeet Ghose, 
  • Pages: 1221-1234
  • First Published: 23 April 2021
Open Access

A useful epitope tag derived from maltose binding protein

Marine Lénon,  Na Ke,  Guoping Ren,  Megan E. Meuser,  Patrick J. Loll,  Paul Riggs,  Mehmet Berkmen, 
  • Pages: 1235-1246
  • First Published: 25 April 2021

PDB Code(s): 7JTR;

Methods and Applications

Free Access

Identifying metal binding amino acids based on backbone geometries as a tool for metalloprotein engineering

Hoang Nguyen,  Jesse Kleingardner, 
  • Pages: 1247-1257
  • First Published: 07 April 2021

Protein Structure Reports

Open Access

Crystal structures of inhibitor complexes of M-PMV protease with visible flap loops

Stanislaw Wosicki,  Maciej Kazmierczyk,  Miroslaw Gilski,  Helena Zabranska,  Iva Pichova,  Mariusz Jaskolski, 
  • Pages: 1258-1263
  • First Published: 31 March 2021

PDB Code(s): 7bgt and 7bgu;

For the Record

Free Access

Criticality of a conserved tyrosine residue in the SpeG protein from Escherichia coli

Van Thi Bich Le,  Joseph Dang,  Ee Qi Lim,  Misty L. Kuhn, 
  • Pages: 1264-1269
  • First Published: 07 April 2021