Red fluorescence of tdT in an RC::FL-tdT/Slc17a6Flpo/+/Phox2bCre/+ embryo at embryonic day 12.5 pointing vagal afferent neurons (see Sesotyosaria et al. pp 205-214)

The official poster for the 1st Asian Xenopus Conference 2024 in Osaka, Japan

The 18th International Zebrafish Conference “IZFC2024” was held in Kyoto from August 17 to 21, 2024.

Ambystoma mexicanum (axolotl) hasrelatively transparent skin, providing an ideal experimental environment forskin biology. However, their skin becomes opaque as they age. This studyfocuses on age-related changes in axolotl skin, including decreased numbers of collagen-producing fibroblasts, altered localization of type III collagen, changes in the thickness of dermal collagen layers, and alterations in fiberpatterns.

We optimized a CRISPR-mediated genome editing method for generating knockin (KI) rats through zygote microinjection, covering zygote harvesting to timed microinjection, which consistently yielded KI rats. Additionally, we established four fluorescent reporter lines at the ROSA26 locus. Our study includes detailed protocols for donor vector design, zygote collection, microinjection, founder screening, and cryopreservation in rats.