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Figure 6 Gel filtration of FXII complexes with polyP and heparin fragments. (a, b, c) Gel-filtration elution profiles for full-length plasma-purified FXII in the presence of increasing molar ratios of polyP with different chain lengths of (a) 34, (b) 55 and (c) 69. Elution volume (Ve) measured in millilitres is shown on the x axis and UV absorption (mAU) is shown on the y axis. (d) Gel-filtration elution profiles for FXII in the presence of increasing molar ratios of heparin fragment Hep20. The second peak arising at a Ve of 1.92 ml is equivalent to the standard gel-filtration standard ferritin, which has a molecular weight of 440 kDa. |